Priming Adipose-Derived Mesenchymal Stem Cells with Hyaluronan Alters Growth Kinetics and Increases Attachment to Articular CartilageReportar como inadecuado

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Stem Cells International - Volume 2016 2016, Article ID 9364213, 13 pages -

Research Article

Department of Chemistry and Biomolecular Sciences, Macquarie University, North Ryde, NSW 2109, Australia

Translational Regenerative Medicine Research Laboratory, Kolling Institute of Medical Research, Institute of Bone and Joint Research, University of Sydney at Royal North Shore Hospital, St Leonards, NSW 2065, Australia

Regeneus Ltd., 25 Bridge Street, Pymble, NSW 2073, Australia

Australian Proteome Analysis Facility, Macquarie University, North Ryde, NSW 2109, Australia

Quirindi Vet Clinic, 81 Pryor Street, Quirindi, NSW 2343, Australia

Received 19 November 2015; Accepted 10 January 2016

Academic Editor: Shinn-Zong Lin

Copyright © 2016 Peter Succar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Biological therapeutics such as adipose-derived mesenchymal stem cell MSC therapy are gaining acceptance for knee-osteoarthritis OA treatment. Reports of OA-patients show reductions in cartilage defects and regeneration of hyaline-like-cartilage with MSC-therapy. Suspending MSCs in hyaluronan commonly occurs in animals and humans, usually without supporting data. Objective. To elucidate the effects of different concentrations of hyaluronan on MSC growth kinetics. Methods. Using a range of hyaluronan concentrations, we measured MSC adherence and proliferation on culture plastic surfaces and a novel cartilage-adhesion assay. We employed time-course and dispersion imaging to assess MSC binding to cartilage. Cytokine profiling was also conducted on the MSC-secretome. Results. Hyaluronan had dose-dependent effects on growth kinetics of MSCs at concentrations of entanglement point 1 mg-mL. At higher concentrations, viscosity effects outweighed benefits of additional hyaluronan. The cartilage-adhesion assay highlighted for the first time that hyaluronan-primed MSCs increased cell attachment to cartilage whilst the presence of hyaluronan did not. Our time-course suggested patients undergoing MSC-therapy for OA could benefit from joint-immobilisation for up to 8 hours. Hyaluronan also greatly affected dispersion of MSCs on cartilage. Conclusion. Our results should be considered in future trials with MSC-therapy using hyaluronan as a vehicle, for the treatment of OA.

Autor: Peter Succar, Michael Medynskyj, Edmond J. Breen, Tony Batterham, Mark P. Molloy, and Benjamin R. Herbert



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