The K–Cl Cotransporter KCC3 as an Independent Prognostic Factor in Human Esophageal Squamous Cell CarcinomaReportar como inadecuado




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BioMed Research InternationalVolume 2014 2014, Article ID 936401, 12 pages

Research Article

Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan

Department of Pathology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan

Departments of Molecular Cell Physiology and Bio-Ionomics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan

Japan Institute for Food Education and Health, St.
Agnes’ University, Kyoto 602-8013, Japan

Received 22 May 2014; Accepted 16 June 2014; Published 9 July 2014

Academic Editor: Akio Tomoda

Copyright © 2014 Atsushi Shiozaki et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objectives of the present study were to investigate the role of K–Cl cotransporter 3 KCC3 in the regulation of cellular invasion and the clinicopathological significance of its expression in esophageal squamous cell carcinoma ESCC.
Immunohistochemical analysis performed on 70 primary tumor samples obtained from ESCC patients showed that KCC3 was primarily found in the cytoplasm of carcinoma cells.
Although the expression of KCC3 in the main tumor MT was related to several clinicopathological features, such as the pT and pN categories, it had no prognostic impact.
KCC3 expression scores were compared between the MT and cancer nest CN, and the survival rate of patients with a score was lower than that of patients with a score.
In addition, the survival rate of patients in whom KCC3 was expressed in the invasive front of tumor was lower than that of the patients without it.
Furthermore, multivariate analysis demonstrated that the expression of KCC3 in the invasive front was one of the most important independent prognostic factors.
The depletion of KCC3 using siRNAs inhibited cell migration and invasion in human ESCC cell lines.
These results suggest that the expression of KCC3 in ESCC may affect cellular invasion and be related to a worse prognosis in patients with ESCC.





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Fuente: https://www.hindawi.com/



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