Mesenchymal stromal cells are more effective than the MSC secretome in diminishing injury and enhancing recovery following ventilator-induced lung injuryReportar como inadecuado




Mesenchymal stromal cells are more effective than the MSC secretome in diminishing injury and enhancing recovery following ventilator-induced lung injury - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Intensive Care Medicine Experimental

, 3:29

First Online: 15 October 2015Received: 23 September 2014Accepted: 14 October 2014

Abstract

BackgroundThe potential for mesenchymal stem cells MSCs to reduce the severity of experimental lung injury has been established in several pre-clinical studies. We have recently demonstrated that MSCs, and MSC-secreted factors secretome, enhance lung repair and regeneration at 48 h following ventilation-induced lung injury VILI. We wished to determine the potential for MSC therapy to exert beneficial effects in the early recovery phase following VILI when ongoing injury coexists with processes of repair, and to compare the efficacy of MSC therapy to the use of the secretome alone.

MethodsMale Sprague–Dawley rats were anesthetized, oro-tracheally intubated, and subjected to high stretch mechanical ventilation until lung compliance had declined by 50 % of baseline. Animals were then weaned from mechanical ventilation, and anesthesia discontinued. Once awake and spontaneously ventilating, animals received an intravenous injection of either rodent MSCs 10 million-kg, MSC-conditioned medium, fibroblasts 10 million-kg, or vehicle. Thereafter, the animals were allowed to recover and the extent of lung injury-repair was determined after 4 h.

ResultsTreatment with MSCs diminished injury and enhanced recovery following VILI to a greater extent compared to MSC-conditioned medium, with fibroblasts proving ineffective. MSCs, but not their conditioned medium, attenuated indices of lung injury including oxygenation, respiratory compliance, and lung edema. Total lung water as assessed by wet:dry ratio, bronchoalveolar lavage total inflammatory cell, neutrophil counts, and alveolar IL-6 concentrations were reduced in the animals that received MSC therapy.

ConclusionsThe immunomodulating and-or reparative effect of MSCs is evident early after VILI in this model. MSC-conditioned medium was not as effective as the cells themselves in diminishing injury and restoring lung structure and function.

KeywordsAcute respiratory distress syndrome Inflammation Ventilation-induced lung injury Repair Mesenchymal stem-stromal cell Mairead Hayes and Gerard F. Curley contributed equally to this work.

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Autor: Mairead Hayes - Gerard F. Curley - Claire Masterson - James Devaney - Daniel O’Toole - John G. Laffey

Fuente: https://link.springer.com/







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