PET Demonstrates Functional Recovery after Treatment by Danhong Injection in a Rat Model of Cerebral Ischemic-Reperfusion InjuryReportar como inadecuado




PET Demonstrates Functional Recovery after Treatment by Danhong Injection in a Rat Model of Cerebral Ischemic-Reperfusion Injury - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Evidence-Based Complementary and Alternative Medicine - Volume 2014 2014, Article ID 430757, 9 pages -

Research Article

Institute of Cardio-Cerebrovascular Diseases, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China

Department of Nuclear Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China

Zhejiang University Medical PET Center, Zhejiang University, Hangzhou 310009, China

Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou 310009, China

Buchang Pharmaceutical Co., Ltd., Xi’an 712000, China

Received 30 September 2013; Revised 13 January 2014; Accepted 17 January 2014; Published 23 February 2014

Academic Editor: Ching-Liang Hsieh

Copyright © 2014 Zefeng Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed to investigate neuroprotection of Danhong injection DHI in a rat model of cerebral ischemia using

F-fluorodeoxyglucose positron emission tomography

F-FDG-PET. Method. Rats were divided into 5 groups: sham group, ischemia-reperfusion untreated IRU group, DHI-1 group DHI 1 mL-kg-d, DHI-2 group DHI 2 mL-kg-d, and DHI-4 group DHI 4 mL-kg-d. AII the treated groups were intraperitoneally injected with DHI daily for 14 days. The therapeutic effects in terms of cerebral infarct volume, neurological function, and cerebral glucose metabolism were evaluated. Expression of TNF- and IL-1 was detected with enzyme-linked immunosorbent assay ELISA. Levels of mature neuronal marker NeuN, glial marker GFAP, vascular density factor vWF, and glucose transporter 1 GLUT1 were assessed by immunohistochemistry. Results. Compared with the IRU group, rats treated with DHI showed dose dependent reductions in cerebral infarct volume and levels of proinflammatory cytokines, improvement of neurological function, and recovery of cerebral glucose metabolism. Meanwhile, the significantly increased numbers of neurons, gliocytes, and vessels and the recovery of glucose utilization were found in the peri-infarct region after DHI treatment using immunohistochemical analysis. Conclusion. This study demonstrated the metabolic recovery after DHI treatment by micro-PET imaging with

F-FDG and the neuroprotective effects of DHI in a rat model of cerebral ischemic-reperfusion injury.





Autor: Zefeng Wang, Fahuan Song, Jinhui Li, Yuyan Zhang, Yu He, Jiehong Yang, Huifen Zhou, Tao Zhao, Wei Fu, Panke Xing, Haiton

Fuente: https://www.hindawi.com/



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