A Nonhost Peptidase Inhibitor of ~14 kDa from Butea monosperma Lam. Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigeraReportar como inadecuado




A Nonhost Peptidase Inhibitor of ~14 kDa from Butea monosperma Lam. Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Biochemistry Research International - Volume 2014 2014, Article ID 361821, 11 pages -

Research Article

Department of Biochemistry DST-FIST & UGC-SAP Supported, Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India

Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh 226025, India

Received 18 January 2014; Accepted 12 March 2014; Published 17 April 2014

Academic Editor: Saad Tayyab

Copyright © 2014 Prabhash K. Pandey et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Helicoverpa armigera is one of the major devastating pests of crop plants. In this context a serine peptidase inhibitor purified from the seeds of Butea monosperma was evaluated for its effect on developmental physiology of H. armigera larvae. B. monosperma peptidase inhibitor on 12% denaturing polyacrylamide gel electrophoresis exhibited a single protein band of ~14 kDa with or without reduction. In vitro studies towards total gut proteolytic enzymes of H. armigera and bovine trypsin indicated measurable inhibitory activity. B. monosperma peptidase inhibitor dose for 50% mortality and weight reduction by 50% were 0.5% w-w and 0.10% w-w, respectively. The IC50 of B. monosperma peptidase inhibitor against total H. armigera gut proteinases activity was 2.0 µg-mL. The larval feeding assays suggested B. monosperma peptidase inhibitor to be toxic as reflected by its retarded growth and development, consequently affecting fertility and fecundity of pest and prolonging the larval-pupal duration of the insect life cycle of H. armigera. Supplementing B. monosperma peptidase inhibitor in artificial diet at 0.1% w-w, both the efficiencies of conversion of ingested as well as digested food were downregulated, whereas approximate digestibility and metabolic cost were enhanced. The efficacy of Butea monosperma peptidase inhibitor against progressive growth and development of H. armigera suggest its usefulness in insect pest management of food crops.





Autor: Prabhash K. Pandey, Dushyant Singh, Sangram Singh, M. Y. Khan, and Farrukh Jamal

Fuente: https://www.hindawi.com/



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