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* Corresponding author 1 Phys-ENS - Laboratoire de Physique de l-ENS Lyon 2 Laboratoire Joliot Curie 3 LBMC - Laboratoire de Biologie Moléculaire de la Cellule 4 INSERM U823, équipe 4 Chromatine et Epigénétique Institut d-oncologie-développement Albert Bonniot de Grenoble

Abstract : The -remodels structure of chromatin- RSC complex is an essential chromatin remodeling factor that is required for the control of several processes including transcription, repair and replication. The ability of RSC to relocate centrally positioned mononucleosomes at the end of nucleosomal DNA is firmly established, but the data on RSC action on oligo-nucleosomal templates remains still scarce. By using atomic force microscopy AFM imaging, we have quantitatively studied the RSC-induced mobilization of positioned di- and trinucleosomes as well as the directionality of mobilization on mononucleosomal template labeled at one end with streptavidin. AFM imaging showed only a limited set of distinct configurational states for the remodeling products. No stepwise or preferred directionality of the nucleosome motion was observed. Analysis of the corresponding reaction pathways allows deciphering the mechanistic features of RSC-induced nucleosome relocation. The final outcome of RSC remodeling of oligosome templates is the packing of the nucleosomes at the edge of the template, providing large stretches of DNA depleted of nucleosomes. This feature of RSC may be used by the cell to overcome the barrier imposed by the presence of nucleosomes.

Keywords : AFM RSC Chromatin Remodeling Dinucleosomes





Autor: Fabien Montel - Martin Castelnovo - Hervé Menoni - Dimitar Angelov - Stefan Dimitrov - Cendrine Faivre-Moskalenko -

Fuente: https://hal.archives-ouvertes.fr/



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