Cellular PathwayS of Antigen Processing and Presentation in Fish APC: Endosomal Involvement and Cell-Free Antigen PresentationReportar como inadecuado




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Developmental Immunology - Volume 3 1992, Issue 1, Pages 51-65



Department of Immunology, Mayo Clinic, Rochester, Minnesota 55905, USA

Department of Microbiology, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA

Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA

Received 20 May 1992; Accepted 2 June 1992

Copyright © 1992 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Studies were conducted to address further the roles of antigen processing andpresentation in the induction of immune responses in a phylogenetically lowervertebrate, specifically a teleost, the channel catfish. In particular, studies were aimed atdetermining the subcellular compartments involved in antigen degradation by channelcatfish antigen-presenting cells APC as well as ascertaining the reexpression ofimmunogenic peptides on the surfaces of APC. The results showed that exogenousprotein antigens were actively endocytosed by APC as detected by flow cytometry. Useof radiolabeled antigen and subcellular fractionation protocols also showed that antigenlocalized in endosomes-lysosomes. Furthermore, there was an apparent redistributionof antigen between these organelles and the plasma membrane during the course ofantigen pulsing. Functional assays for the induction of in vitro antigen-specificproliferation of immune catfish peripheral blood leukocytes PBL showed thatmembrane preparations from antigen-pulsed autologous APC were highly stimulatory.The magnitude of responses elicited with such membrane preparations was very similarto that of PBL cultures stimulated with native antigen-pulsed and fixed intact APC orprefixed intact APC incubated with a peptide fragment of the nominal antigen. Currentdata further corroborate our previous findings that steps akin to antigen processing andpresentation are clearly important in the induction of immune responses in lowervertebrates like fish, in a manner similar to that seen in mammalian systems.Consequently, it would appear that many immune functions among the diverse taxa ofvertebrates are remarkably conserved.





Autor: Abbe N. Vallejo, Norman W. Miller, Nancy E. Harvey, Marvin A. Cuchens, Gregory W. Warr, and L. William Clem

Fuente: https://www.hindawi.com/



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