Evolutionary genomics of mycovirus-related dsRNA viruses reveals cross-family horizontal gene transfer and evolution of diverse viral lineagesReportar como inadecuado

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BMC Evolutionary Biology

, 12:91

First Online: 20 June 2012Received: 16 March 2012Accepted: 06 June 2012DOI: 10.1186-1471-2148-12-91

Cite this article as: Liu, H., Fu, Y., Xie, J. et al. BMC Evol Biol 2012 12: 91. doi:10.1186-1471-2148-12-91


BackgroundDouble-stranded ds RNA fungal viruses are typically isometric single-shelled particles that are classified into three families, Totiviridae, Partitiviridae and Chrysoviridae, the members of which possess monopartite, bipartite and quadripartite genomes, respectively. Recent findings revealed that mycovirus-related dsRNA viruses are more diverse than previously recognized. Although an increasing number of viral complete genomic sequences have become available, the evolution of these diverse dsRNA viruses remains to be clarified. This is particularly so since there is little evidence for horizontal gene transfer HGT among dsRNA viruses.

ResultsIn this study, we report the molecular properties of two novel dsRNA mycoviruses that were isolated from a field strain of Sclerotinia sclerotiorum, Sunf-M: one is a large monopartite virus representing a distinct evolutionary lineage of dsRNA viruses; the other is a new member of the family Partitiviridae. Comprehensive phylogenetic analysis and genome comparison revealed that there are at least ten monopartite, three bipartite, one tripartite and three quadripartite lineages in the known dsRNA mycoviruses and that the multipartite lineages have possibly evolved from different monopartite dsRNA viruses. Moreover, we found that homologs of the S7 Domain, characteristic of members of the genus phytoreovirus in family Reoviridae are widely distributed in diverse dsRNA viral lineages, including chrysoviruses, endornaviruses and some unclassified dsRNA mycoviruses. We further provided evidence that multiple HGT events may have occurred among these dsRNA viruses from different families.

ConclusionsOur study provides an insight into the phylogeny and evolution of mycovirus-related dsRNA viruses and reveals that the occurrence of HGT between different virus species and the development of multipartite genomes during evolution are important macroevolutionary mechanisms in dsRNA viruses.

AbbreviationsAaAmino acid

aLRTApproximate likelihood ratio test

BLASTBasic Local Alignment Search Tool

CDDConserved Domain Database

CPCapsid protein


HGTHorizontal gene transfer

MCMCsMonte Carlo Markov Chains


NCBINational Center for Biotechnology Information

ORFsOpen reading frames

RdRpRNA-dependent RNA polymerase


Electronic supplementary materialThe online version of this article doi:10.1186-1471-2148-12-91 contains supplementary material, which is available to authorized users.

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Autor: Huiquan Liu - Yanping Fu - Jiatao Xie - Jiasen Cheng - Said A Ghabrial - Guoqing Li - Youliang Peng - Xianhong Yi - Daoho

Fuente: https://link.springer.com/

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