Phylogeography of Camellia taliensis Theaceae inferred from chloroplast and nuclear DNA: insights into evolutionary history and conservationReportar como inadecuado




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BMC Evolutionary Biology

, 12:92

First Online: 21 June 2012Received: 08 July 2011Accepted: 21 June 2012DOI: 10.1186-1471-2148-12-92

Cite this article as: Liu, Y., Yang, S., Ji, P. et al. BMC Evol Biol 2012 12: 92. doi:10.1186-1471-2148-12-92

Abstract

BackgroundAs one of the most important but seriously endangered wild relatives of the cultivated tea, Camellia taliensis harbors valuable gene resources for tea tree improvement in the future. The knowledge of genetic variation and population structure may provide insights into evolutionary history and germplasm conservation of the species.

ResultsHere, we sampled 21 natural populations from the species- range in China and performed the phylogeography of C. taliensis by using the nuclear PAL gene fragment and chloroplast rpl32-trnL intergenic spacer. Levels of haplotype diversity and nucleotide diversity detected at rpl32-trnL h = 0.841; π = 0.00314 were almost as high as at PAL h = 0.836; π = 0.00417. Significant chloroplast DNA population subdivision was detected GST = 0.988; NST = 0.989, suggesting fairly high genetic differentiation and low levels of recurrent gene flow through seeds among populations. Nested clade phylogeographic analysis of chlorotypes suggests that population genetic structure in C. taliensis has been affected by habitat fragmentation in the past. However, the detection of a moderate nrDNA population subdivision GST = 0.222; NST = 0.301 provided the evidence of efficient pollen-mediated gene flow among populations and significant phylogeographical structure NST > GST; P < 0.01. The analysis of PAL haplotypes indicates that phylogeographical pattern of nrDNA haplotypes might be caused by restricted gene flow with isolation by distance, which was also supported by Mantel’s test of nrDNA haplotypes r = 0.234, P < 0.001. We found that chlorotype C1 was fixed in seven populations of Lancang River Region, implying that the Lancang River might have provided a corridor for the long-distance dispersal of the species.

ConclusionsWe found that C. taliensis showed fairly high genetic differentiation resulting from restricted gene flow and habitat fragmentation. This phylogeographical study gives us deep insights into population structure of the species and conservation strategies for germplasm sampling and developing in situ conservation of natural populations.

AbbreviationsAMOVAAnalysis of molecular variance

NCPANested clade phylogeographic analysis

PALPhenylalanine ammonia-lyase

SNPSingle nucleotide polymorphisms.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2148-12-92 contains supplementary material, which is available to authorized users.

Yang Liu, Shi-xiong Yang contributed equally to this work.

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Autor: Yang Liu - Shi-xiong Yang - Peng-zhang Ji - Li-zhi Gao

Fuente: https://link.springer.com/



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