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BMC Microbiology

, 12:144

First Online: 20 July 2012Received: 10 February 2012Accepted: 20 July 2012DOI: 10.1186-1471-2180-12-144

Cite this article as: Pushalkar, S., Ji, X., Li, Y. et al. BMC Microbiol 2012 12: 144. doi:10.1186-1471-2180-12-144


BackgroundBacterial infections have been linked to malignancies due to their ability to induce chronic inflammation. We investigated the association of oral bacteria in oral squamous cell carcinoma OSCC-tumor tissues and compared with adjacent non-tumor mucosa sampled 5 cm distant from the same patient n = 10. By using culture-independent 16S rRNA approaches, denaturing gradient gel electrophoresis DGGE and cloning and sequencing, we assessed the total bacterial diversity in these clinical samples.

ResultsDGGE fingerprints showed variations in the band intensity profiles within non-tumor and tumor tissues of the same patient and among the two groups. The clonal analysis indicated that from a total of 1200 sequences characterized, 80 bacterial species-phylotypes were detected representing six phyla, Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria, Actinobacteria and uncultivated TM7 in non-tumor and tumor libraries. In combined library, 12 classes, 16 order, 26 families and 40 genera were observed. Bacterial species, Streptococcus sp. oral taxon 058, Peptostreptococcus stomatis, Streptococcus salivarius, Streptococcus gordonii, Gemella haemolysans, Gemella morbillorum, Johnsonella ignava and Streptococcus parasanguinis I were highly associated with tumor site where as Granulicatella adiacens was prevalent at non-tumor site. Streptococcus intermedius was present in 70% of both non-tumor and tumor sites.

ConclusionsThe underlying changes in the bacterial diversity in the oral mucosal tissues from non-tumor and tumor sites of OSCC subjects indicated a shift in bacterial colonization. These most prevalent or unique bacterial species-phylotypes present in tumor tissues may be associated with OSCC and needs to be further investigated with a larger sample size.

KeywordsMicrobial diversity Oral squamous cell carcinoma Microbiota Tumor Non-tumor Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-12-144 contains supplementary material, which is available to authorized users.

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Autor: Smruti Pushalkar - Xiaojie Ji - Yihong Li - Cherry Estilo - Ramanathan Yegnanarayana - Bhuvanesh Singh - Xin Li - Deepak S


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