Individual CLA Isomers, c9t11 and t10c12, Prevent Excess Liver Glycogen Storage and Inhibit Lipogenic Genes Expression Induced by High-Fructose Diet in RatsReportar como inadecuado




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BioMed Research International - Volume 2015 2015, Article ID 535982, 10 pages -

Research Article

Department of Human Nutrition, Faculty of Food Technology, Agricultural University of Krakow, Balicka 122, 30-149 Krakow, Poland

Jagiellonian Centre for Experimental Therapeutics JCET, Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland

Department of Chemistry, Faculty of Food Science, Wroclaw University of Environmental and Life Sciences, C. K. Norwida 25, 50-375 Wroclaw, Poland

Department of Clinical and Experimental Pathomorphology, Jagiellonian University Medical College, Grzegorzecka 16, 31-531 Krakow, Poland

Department of Experimental Pharmacology, Jagiellonian University Medical College, Grzegorzecka 16, 31-531 Krakow, Poland

Received 10 October 2014; Revised 12 January 2015; Accepted 26 February 2015

Academic Editor: Maria J. Martins

Copyright © 2015 Edyta Maslak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study assessed the effects of individual conjugated linoleic acid isomers, c9t11-CLA and t10c12-CLA, on nonalcoholic fatty liver disease NAFLD and systemic endothelial dysfunction in rats fed for four weeks with control or high-fructose diet. The high-fructose diet hampered body weight gain without influencing food intake, increased liver weight and glycogen storage in hepatocytes, upregulated expression of fatty acid synthase FAS and stearoyl-CoA desaturase-1 SCD-1, and increased saturated fatty acid SFA content in the liver. Both CLA isomers prevented excessive accumulation of glycogen in the liver. Specifically, t10c12-CLA decreased concentration of serum triacylglycerols and LDL + VLDL cholesterol, increased HDL cholesterol, and affected liver lipid content and fatty acid composition by downregulation of liver SCD-1 and FAS expression. In turn, the c9t11-CLA decreased LDL+VLDL cholesterol in the control group and downregulated liver expression of FAS without significant effects on liver weight, lipid content, and fatty acid composition. In summary, feeding rats with a high-fructose diet resulted in increased liver glycogen storage, indicating the induction of gluconeogenesis despite simultaneous upregulation of genes involved in de novo lipogenesis. Although both CLA isomers c9t11 and t10c12 display hepatoprotective activity, the hypolipemic action of the t10c12-CLA isomer proved to be more pronounced than that of c9t11-CLA.





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Fuente: https://www.hindawi.com/



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