Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stressReport as inadecuate

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BMC Genomics

, 13:336

First Online: 23 July 2012Received: 02 December 2011Accepted: 01 July 2012DOI: 10.1186-1471-2164-13-336

Cite this article as: Yang, S., Giannone, R.J., Dice, L. et al. BMC Genomics 2012 13: 336. doi:10.1186-1471-2164-13-336


BackgroundClostridium thermocellum is a candidate consolidated bioprocessing biocatalyst, which is a microorganism that expresses enzymes for both cellulose hydrolysis and its fermentation to produce fuels such as lignocellulosic ethanol. However, C. thermocellum is relatively sensitive to ethanol compared to ethanologenic microorganisms such as yeast and Zymomonas mobilis that are used in industrial fermentations but do not possess native enzymes for industrial cellulose hydrolysis.

ResultsIn this study, C. thermocellum was grown to mid-exponential phase and then treated with ethanol to a final concentration of 3.9 g-L to investigate its physiological and regulatory responses to ethanol stress. Samples were taken pre-shock and 2, 12, 30, 60, 120, and 240 min post-shock, and from untreated control fermentations for systems biology analyses. Cell growth was arrested by ethanol supplementation with intracellular accumulation of carbon sources such as cellobiose, and sugar phosphates, including fructose-6-phosphate and glucose-6-phosphate. The largest response of C. thermocellum to ethanol shock treatment was in genes and proteins related to nitrogen uptake and metabolism, which is likely important for redirecting the cells physiology to overcome inhibition and allow growth to resume.

ConclusionThis study suggests possible avenues for metabolic engineering and provides comprehensive, integrated systems biology datasets that will be useful for future metabolic modeling and strain development endeavors.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-13-336 contains supplementary material, which is available to authorized users.

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Author: Shihui Yang - Richard J Giannone - Lezlee Dice - Zamin K Yang - Nancy L Engle - Timothy J Tschaplinski - Robert L Hett


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