Expression of human growth hormone in the milk of transgenic rabbits with transgene mapped to the telomere region of chromosome 7qReportar como inadecuado




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Journal of Applied Genetics

, Volume 53, Issue4, pp 435442

First Online: 17 August 2012Received: 16 March 2012Revised: 29 June 2012Accepted: 30 July 2012DOI: 10.1007-s13353-012-0110-4

Cite this article as: Lipinski, D., Zeyland, J., Szalata, M.
et al.
J Appl Genetics 2012 53: 435.
doi:10.1007-s13353-012-0110-4

Abstract

The advent of transgenic technology has provided methods for the production of pharmaceuticals by the isolation of these proteins from transgenic animals.
The mammary gland has been focused on as a bioreactor, since milk is easily collected from lactating animals and protein production can be expressed at very high levels, including hormones and enzymes.
We demonstrate here the expression pattern of recombinant human growth hormone rhGH in transgenic rabbits carrying hGH genomic sequences driven by the rat whey acidic protein WAP promoter.
The transgene was mapped to the q26-27 telomere region of chromosome 7q by fluorescence in situ hybridization FISH.
Nearly 30 % of the F1 generation demonstrated the presence of transgene.
The recombinant growth hormone was detected in the milk of the transgenic rabbit females, but not in serum, up to the level of 10g-ml.
Ectopic expression of the transgene in the brain, heart, kidney, liver, and salivary gland was not observed, indicating that a short sequence of rat WAP promoter 969 bp contained essential sequences directing expression exclusively to the mammary gland.
The biological activity of recombinant growth hormone was measured by immunoreactivity and the capability to stimulate growth of the hormone-dependent Nb211 cell line.

KeywordsWhey acidic protein promoterRecombinant human growth hormoneTransgenic animals Download fulltext PDF



Autor: DanielLipinski - JoannaZeyland - MarlenaSzalata - AndrzejPlawski - MalgorzataJarmuz - JacekJura - AleksandraKorcz - ZdzislawSmorag

Fuente: https://link.springer.com/



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