Enhancement of the efficiency of PEI-liposome transfection by magnetofectins formed via electrostatic self-assemblyReportar como inadecuado




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Chinese Science Bulletin

, Volume 57, Issue31, pp 40054011

First Online: 14 May 2012Received: 10 October 2011Accepted: 03 February 2012DOI: 10.1007-s11434-012-5184-1

Cite this article as: Ma, Y., Wang, X. & Gu, H. Chin. Sci. Bull. 2012 57: 4005. doi:10.1007-s11434-012-5184-1

Abstract

One of the major challenges for successful gene therapy is improving the transfection efficiency of non-viral vectors. Magnetic nanoparticles MNPs have been developed as enhancers of non-viral vehicles. We prepared MNPs and modified them with polyethyleneimine PEI, citric acid CA or carboxylmethyl-dextran CMD. Both positively charged MNPs MNPs@PEI and negatively charged MNPs MNPs@CA, MNPs@CMD could spontaneously form transfection complexes magnetofectins with plasmid DNA and PEI-liposome via electrostatic self-assembly. Our results showed as-prepared magnetofectins apparently enhanced PEI-liposome transfection efficiency and-or gene expression level into COS-7 cells with reduced transfection time from 4 h to 15 min under a magnetic field in vitro. Meanwhile, the effect of magnetofection was cell line-dependant. These results suggest that charged MNPs could improve transfection efficiency for non-viral vectors by simply mixing with them and by exerting a magnetic force. Thus such MNPs provide a convenient platform for further applications of gene delivery.

Keywordsgene deliveryliposomemagnetofectionmagnetic nanoparticlesPEIThis article is published with open access at Springerlink.com

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Autor: YongJieMa - XiaoLiangWang - HongChenGu

Fuente: https://link.springer.com/







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