PRMT5 suppresses DR4-mediated CCL20 release via NF-B pathwayReportar como inadecuado

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Chinese Science Bulletin

, Volume 57, Issue33, pp 43514355

First Online: 13 November 2012Received: 27 December 2011Accepted: 08 February 2012DOI: 10.1007-s11434-012-5511-6

Cite this article as: Wang, D., Liu, D., Gao, J. et al. Chin. Sci. Bull. 2012 57: 4351. doi:10.1007-s11434-012-5511-6


Construct expression vectors of pCMV-DR4-HA and pCMV-PRMT5-Flag, and transfect them into HEK293 cells to identify the interaction between TRAIL-R1 and PRMT5 and the molecular mechanism underlying DR4-mediated inhibition of chemokine CCL20 release via TRAIL receptor 1 DR4. Inflammatory cytokine was detected by RT-PCR and ELISA after TRAIL-R1 and-or PRMT5 transfection, respectively. NF-B activity was detected by Dual Luciferase Reporter Gene Assay. ERK1-2 phosphorylation was analyzed by Western blot. PRMT5 could inhibit DR4-activated NF-B activity and ERK1-2 phosphorylation. PRMT5 could inhibit NF-B activition, ERK1-2 phosphorylation as well as CCL20 secretion via binding with DR4 in HEK293 cell, suggesting that PRMT5 may involve in DR4 dependent immune regulation.

KeywordsTRAILPRMT5cytokineCCL20This article is published with open access at

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Autor: DongShengWang - DanLiu - JingGao - MinLiu - ShiLianLiu - YanXinLiu - DeXianZheng


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