Protective Effect of Danhong Injection on Acute Hepatic Failure Induced by Lipopolysaccharide and D-Galactosamine in MiceReportar como inadecuado

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Evidence-Based Complementary and Alternative Medicine - Volume20142014, Article ID153902, 8 pages -

Research ArticleTianjin State Key Laboratory of Modern Chinese Medicine, Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, No. 88 Yuquan Road, Nankai District, Tianjin 300193, China

Received 29 October 2013; Revised 24 January 2014; Accepted 11 February 2014; Published 17 March 2014

Academic Editor: K. N. S.Sirajudeen

Copyright 2014 Ying Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Acute hepatic failure AHF, which leads to an extremely high mortality rate, has become the focus of attention in clinic. In this study, Danhong injection DHI was investigated to evaluate the preventive and protective effect on AHF induced by lipopolysaccharide LPS and D-galactosamine GalN in mice. For AHF induction, ICR mice were intraperitoneally injected with D-GalN 700mg-kg and LPS 20g-kg. DHI was administrated twice, at 12 and 1h, respectively, before D-GalN-LPS injection. After stimulation with D-GalN-LPS for 1 and 6h, serum and livers were collected for analysis. We found that mice administrated with DHI displayed a higher survival rate, lower serum levels of alanine aminotransferase ALT, aspartate aminotransferase AST, total bilirubin TBil, glutathione S-transferase GST, and tumor necrosis factor TNF-. DHI inhibited the elevations of hepatic lipid peroxidation malondialdehyde, caspase-8 activity, and mRNA expression levels of inflammatory cytokines interleukin-1 and interleukin-6 increased by D-GalN-LPS in the liver. Furthermore, liver histopathological analysis indicated that the DHI group showed markedly fewer apoptotic TUNEL positive cells and less pathological changes than those in the AHF model group. These results provide a novel insight into the pharmacological actions of DHI as a potential candidate for treating AHF.

Autor: Ying Wang,Li-Na Gao,Yuan-Lu Cui,and Heng-Li Jiang



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