Global gene expression of the inner cell mass and trophectoderm of the bovine blastocystReport as inadecuate

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BMC Developmental Biology

, 12:33

Early development


BackgroundThe first distinct differentiation event in mammals occurs at the blastocyst stage when totipotent blastomeres differentiate into either pluripotent inner cell mass ICM or multipotent trophectoderm TE. Here we determined, for the first time, global gene expression patterns in the ICM and TE isolated from bovine blastocysts. The ICM and TE were isolated from blastocysts harvested at day 8 after insemination by magnetic activated cell sorting, and cDNA sequenced using the SOLiD 4.0 system.

ResultsA total of 870 genes were differentially expressed between ICM and TE. Several genes characteristic of ICM for example, NANOG, SOX2, and STAT3 and TE ELF5, GATA3, and KRT18 in mouse and human showed similar patterns in bovine. Other genes, however, showed differences in expression between ICM and TE that deviates from the expected based on mouse and human.

ConclusionAnalysis of gene expression indicated that differentiation of blastomeres of the morula-stage embryo into the ICM and TE of the blastocyst is accompanied by differences between the two cell lineages in expression of genes controlling metabolic processes, endocytosis, hatching from the zona pellucida, paracrine and endocrine signaling with the mother, and genes supporting the changes in cellular architecture, stemness, and hematopoiesis necessary for development of the trophoblast.

KeywordsBlastocystTrophectodermInner cell massDevelopmentAbbreviationsDAVIDDatabase for annotation, visualization and integrated discovery

GOGene ontology

KEGGKyoto encyclopedia of genes and genomes

ICMInner cell mass

SSESum of squares error

TFBSTranscription factor binding sites


Electronic supplementary materialThe online version of this article doi:10.1186-1471-213X-12-33 contains supplementary material, which is available to authorized users.

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Author: ManabuOzawa - MikiSakatani - JiQiangYao - SavitaShanker - FahongYu - RuiYamashita - ShunichiWakabayashi - KentaNakai - KyleBDobbs


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