Whole genome sequencing of the fish pathogen Francisella noatunensis subsp. orientalis Toba04 gives novel insights into Francisella evolution and pathogenecityReportar como inadecuado




Whole genome sequencing of the fish pathogen Francisella noatunensis subsp. orientalis Toba04 gives novel insights into Francisella evolution and pathogenecity - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Genomics

, 13:598

Prokaryote microbial genomics

Abstract

BackgroundFrancisella is a genus of gram-negative bacterium highly virulent in fishes and human where F.
tularensis is causing the serious disease tularaemia in human.
Recently Francisella species have been reported to cause mortality in aquaculture species like Atlantic cod and tilapia.
We have completed the sequencing and draft assembly of the Francisella noatunensis subsp.
orientalisToba04 strain isolated from farmed Tilapia.
Compared to other available Francisella genomes, it is most similar to the genome of Francisella philomiragia subsp.
philomiragia, a free-living bacterium not virulent to human.

ResultsThe genome is rearranged compared to the available Francisella genomes even though we found no IS-elements in the genome.
Nearly 16% percent of the predicted ORFs are pseudogenes.
Computational pathway analysis indicates that a number of the metabolic pathways are disrupted due to pseudogenes.
Comparing the novel genome with other available Francisella genomes, we found around 2.5% of unique genes present in Francisella noatunensis subsp.
orientalis Toba04 and a list of genes uniquely present in the human-pathogenic Francisella subspecies.
Most of these genes might have transferred from bacterial species through horizontal gene transfer.
Comparative analysis between human and fish pathogen also provide insights into genes responsible for pathogenecity.
Our analysis of pseudogenes indicates that the evolution of Francisella subspeciess pseudogenes from Tilapia is old with large number of pseudogenes having more than one inactivating mutation.

ConclusionsThe fish pathogen has lost non-essential genes some time ago.
Evolutionary analysis of the Francisella genomes, strongly suggests that human and fish pathogenic Francisella species have evolved independently from free-living metabolically competent Francisella species.
These findings will contribute to understanding the evolution of Francisella species and pathogenesis.

KeywordsFrancisellaMacrophagePseudogenesGenomeInsertion elementsComparative analysisElectronic supplementary materialThe online version of this article doi:10.1186-1471-2164-13-598 contains supplementary material, which is available to authorized users.

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Autor: SettuSridhar - AnimeshSharma - HeidiKongshaug - FrankNilsen - IngeJonassen

Fuente: https://link.springer.com/



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