B chromosome in the beetle Coprophanaeus cyanescens Scarabaeidae: emphasis in the organization of repetitive DNA sequencesReportar como inadecuado

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BMC Genetics

, 13:96

Epigenetics and chromosome biology


BackgroundTo contribute to the knowledge of coleopteran cytogenetics, especially with respect to the genomic content of B chromosomes, we analyzed the composition and organization of repetitive DNA sequences in the Coprophanaeus cyanescens karyotype. We used conventional staining and the application of fluorescence in situ hybridization FISH mapping using as probes C0t-1 DNA fraction, the 18S and 5S rRNA genes, and the LOA-like non-LTR transposable element TE.

ResultsThe conventional analysis detected 3 individuals among 50 analyzed carrying one small metacentric and mitotically unstable B chromosome. The FISH analysis revealed a pericentromeric block of C0t-1 DNA in the B chromosome but no 18S or 5S rDNA clusters in this extra element. Using the LOA-like TE probe, the FISH analysis revealed large pericentromeric blocks in eight autosomal bivalents and in the B chromosome, and a pericentromeric block extending to the short arm in one autosomal pair. No positive hybridization signal was observed for the LOA-like element in the sex chromosomes.

ConclusionsThe results indicate that the origin of the B chromosome is associated with the autosomal elements, as demonstrated by the hybridization with C0t-1 DNA and the LOA-like TE. The present study is the first report on the cytogenetic mapping of a TE in coleopteran chromosomes. These TEs could have been involved in the origin and evolution of the B chromosome in C. cyanescens.

KeywordsChromosomal rearrangementsHeterochromatinMultigene familiesSupernumerary chromosomeTransposable elementsAbbreviationsCEEAComisso de tica em Experimentao Animal

DAPI4- 6-Diamidino-2-Phenylindole

FISHFluorescence In Situ Hybridization

IBAMAInstituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renovveis

IBBInstituto de Biocincias de Botucatu

LIRMMLaboratoire Le dInformatique, Robotiqueet de Microlectronique of Montpellier

LTRLong Terminal Repeat

MMAMinistrio do Meio Ambiente

NCBINational Center for Biotechnology Information

PCRPolymerase Chain Reaction

rDNAribosomal DNA

rRNAribosomal RNA

SISBIOSistema de Autorizao e Informao em Biodiversidade

UNESPUniversidade Estadual Paulista

TEsTransposable Elements.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2156-13-96 contains supplementary material, which is available to authorized users.

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Autor: SarahGomes de Oliveira - RitaCassia de Moura - CesarMartins

Fuente: https://link.springer.com/

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