Updated 16S rRNA-RFLP method for the identification of all currently characterised ArcobactersppReportar como inadecuado




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BMC Microbiology

, 12:292

First Online: 18 December 2012Received: 18 May 2012Accepted: 13 December 2012DOI: 10.1186-1471-2180-12-292

Cite this article as: Figueras, M.J., Levican, A. & Collado, L. BMC Microbiol 2012 12: 292. doi:10.1186-1471-2180-12-292

Abstract

BackgroundArcobacter spp. family Campylobacteraceae are ubiquitous zoonotic bacteria that are being increasingly recognised as a threat to human health. A previously published 16S rRNA-RFLP Arcobacter spp. identification method produced specific RFLP patterns for the six species described at that time, using a single endonuclease MseI. The number of characterised Arcobacter species has since risen to 17. The aim of the present study was to update the 16S rRNA-RFLP identification method to include all currently characterised species of Arcobacter.

ResultsDigestion of the 16S rRNA gene with the endonuclease MseI produced clear, distinctive patterns for 10 of the 17 species, while the remaining species shared a common or very similar RFLP pattern. Subsequent digestion of the 16S rRNA gene from these species with the endonucleases MnlI and-or BfaI generated species-specific RFLP patterns.

Conclusions16S rRNA-RFLP analysis identified 17 Arcobacter spp. using either polyacrylamide or agarose gel electrophoresis. Microheterogeneities within the 16S rRNA gene, which interfered with the RFLP identification, were also documented for the first time in this genus, particularly in strains of Arcobacter cryaerophilus isolated from animal faeces and aborted foetuses.

KeywordsArcobacterIdentificationAgarosePolyacrylamide16S rRNA-RFLP16S rRNA gene mutationsElectronic supplementary materialThe online version of this article doi:10.1186-1471-2180-12-292 contains supplementary material, which is available to authorized users.

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Autor: MaraJosFigueras - ArturoLevican - LuisCollado

Fuente: https://link.springer.com/







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