Characterization of a newly identified rice chitinase-like protein OsCLP homologous to xylanase inhibitorReport as inadecuate

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BMC Biotechnology

, 13:4

Plant biotechnology


BackgroundDuring rice blast fungal attack, plant xylanase inhibitor proteins XIPs that inhibit fungal xylanase activity are believed to act as a defensive barrier against fungal pathogens. To understand the role of XIPs in rice, a xylanase inhibitor was cloned from rice. The expression of this gene was examined at the transcriptional-translational levels during compatible and incompatible interactions, and the biochemical activity of this protein was also examined.

ResultsSequence alignment revealed that the deduced amino acid sequence of OsCLP shares a high degree of similarity with that of other plant TAXI-type XIPs. However, recombinant OsCLP did not display inhibitory activity against endo-1,4-β-xylanase enzymes from Aureobasidium pullulans A. pullulans or Trichoderma viride T. viride. Instead, an in-gel activity assay revealed strong chitinase activity. The transcription and translation of OsCLP were highly induced when rice was exposed to pathogens in an incompatible interaction. In addition, exogenous treatment with OsCLP affected the growth of the basidiomycete fungus Rhizoctonia solani through degradation of the hyphal cell wall. These data suggest that OsCLP, which has chitinase activity, may play an important role in plant defenses against pathogens.

ConclusionsTaken together, our results demonstrate that OsCLP may have antifungal activity. This protein may directly inhibit pathogen growth by degrading fungal cell wall components through chitinase activity.

KeywordsAntifungal activity Chitinase Oryza sativa Xylanase inhibitor Electronic supplementary materialThe online version of this article doi:10.1186-1472-6750-13-4 contains supplementary material, which is available to authorized users.

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Author: Jingni Wu - Yiming Wang - Sun Tae Kim - Sang Gon Kim - Kyu Young Kang


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