Detection limits of Legionella pneumophila in environmental samples after co-culture with Acanthamoeba polyphagaReportar como inadecuado




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BMC Microbiology

, 13:49

Ecological and evolutionary microbiology

Abstract

BackgroundThe efficiency of recovery and the detection limit of Legionella after co-culture with Acanthamoeba polyphaga are not known and so far no investigations have been carried out to determine the efficiency of the recovery of Legionella spp. by co-culture and compare it with that of conventional culturing methods. This study aimed to assess the detection limits of co-culture compared to culture for Legionella pneumophila in compost and air samples. Compost and air samples were spiked with known concentrations of L. pneumophila. Direct culturing and co-culture with amoebae were used in parallel to isolate L. pneumophila and recovery standard curves for both methods were produced for each sample.

ResultsThe co-culture proved to be more sensitive than the reference method, detecting 10-10 L. pneumophila cells in 1 g of spiked compost or 1 m of spiked air, as compared to 1010 cells in 1 g of spiked compost and 1 m of spiked air.

ConclusionsCo-culture with amoebae is a useful, sensitive and reliable technique to enrich L. pneumophila in environmental samples that contain only low amounts of bacterial cells.

KeywordsLegionella Culture Co-culture Compost Air Bioaerosol Detection limit Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-13-49 contains supplementary material, which is available to authorized users.

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Autor: Lisa Conza - Simona Casati - Valeria Gaia

Fuente: https://link.springer.com/







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