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BMC Genomics

, 14:215

Human and rodent genomics

Abstract

BackgroundMammalian genes are regulated through the action of multiple regulatory elements, often distributed across large regions. The mechanisms that control the integration of these diverse inputs into specific gene expression patterns are still poorly understood. New approaches enabling the dissection of these mechanisms in vivo are needed.

ResultsHere, we describe TRACER http:-tracerdatabase.embl.de, a resource that centralizes information from a large on-going functional exploration of the mouse genome with different transposon-associated regulatory sensors. Hundreds of insertions have been mapped to specific genomic positions, and their corresponding regulatory potential has been documented by analysis of the expression of the reporter sensor gene in mouse embryos. The data can be easily accessed and provides information on the regulatory activities present in a large number of genomic regions, notably in gene-poor intervals that have been associated with human diseases.

ConclusionsTRACER data enables comparisons with the expression pattern of neighbouring genes, activity of surrounding regulatory elements or with other genomic features, revealing the underlying regulatory architecture of these loci. TRACER mouse lines can also be requested for in vivo transposition and chromosomal engineering, to analyse further regions of interest.

KeywordsGene regulation and expression Genome organisation Regulatory landscapes Chromosomal engineering Mouse models of human structural variation Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-14-215 contains supplementary material, which is available to authorized users.

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Autor: Chao-Kung Chen - Orsolya Symmons - Veli Vural Uslu - Taro Tsujimura - Sandra Ruf - Damian Smedley - François Spitz

Fuente: https://link.springer.com/







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