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BMC Genomics

, 14:312

Prokaryote microbial genomics


BackgroundProbiotic bifidobacteria in combination with prebiotic carbohydrates have documented positive effects on human health regarding gastrointestinal disorders and improved immunity, however the selective routes of uptake remain unknown for most candidate prebiotics. The differential transcriptomes of Bifidobacterium animalis subsp. lactis Bl-04, induced by 11 potential prebiotic oligosaccharides were analyzed to identify the genetic loci involved in the uptake and catabolism of α- and β-linked hexoses, and β-xylosides.

ResultsThe overall transcriptome was modulated dependent on the type of glycoside galactosides, glucosides or xylosides utilized. Carbohydrate transporters of the major facilitator superfamily induced by gentiobiose and β-galacto-oligosaccharides GOS and ATP-binding cassette ABC transporters upregulated by cellobiose, GOS, isomaltose, maltotriose, melibiose, panose, raffinose, stachyose, xylobiose and β-xylo-oligosaccharides were differentially upregulated, together with glycoside hydrolases from families 1, 2, 13, 36, 42, 43 and 77. Sequence analysis of the identified solute-binding proteins that determine the specificity of ABC transporters revealed similarities in the breadth and selectivity of prebiotic utilization by bifidobacteria.

ConclusionThis study identified the differential gene expression for utilization of potential prebiotics highlighting the extensive capabilities of Bifidobacterium lactis Bl-04 to utilize oligosaccharides. Results provide insights into the ability of this probiotic microbe to utilize indigestible carbohydrates in the human gastrointestinal tract.

KeywordsBifidobacterium lactis Transcriptomics ABC transporter GPH transporter Prebiotics Glycoside hydrolase AbbreviationsANOVAAnalysis of variance

ABCATP-binding cassette


COGClusters of Orthologous Groups

GHGlycoside hydrolase

MFSMajor Facilitator Superfamily

RFORaffinose family oligosaccharides


Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-14-312 contains supplementary material, which is available to authorized users.

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