Effects of Cobalt on Membrane ATPases, Oxidant, and Antioxidant Values in the Cerebrum and Cerebellum of Suckling RatsReportar como inadecuado




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Biological Trace Element Research

, Volume 154, Issue 3, pp 387–395

First Online: 16 July 2013Received: 25 January 2013Accepted: 25 June 2013DOI: 10.1007-s12011-013-9746-0

Cite this article as: Garoui, E., Amara, I.B., Driss, D. et al. Biol Trace Elem Res 2013 154: 387. doi:10.1007-s12011-013-9746-0

Abstract

Chronic overexposure to cobalt Co may result in neurotoxic effects, but the mechanism of Co-induced neurotoxicity is not yet well established. Our study was conducted to determine whether Co is associated to the induction of central nervous system damage in pregnant rats and their progeny. Twelve pregnant female rats were randomly divided into 2 groups: group I served as controls and group II received Co 350 mg-L, orally. Treatments started from the 14th day of pregnancy until day 14 after delivery. Co concentration in plasma was higher in the treated groups than in the controls. Exposure to Co also increased the levels of MDA, PCO, H2O2, and AOPP, while NaK-ATPase and Mg-ATPase, AChE, and BuChE activities decreased in the cerebrum and cerebellum of suckling pups. A smear without ladder formation on agarose gel was also shown in the cerebrum and cerebellum, indicating random DNA degradation. A reduction in GPx, SOD, CAT, GSH, NPSH, and vitamin C values was observed. The changes were confirmed by histological results. In conclusion, these data showed that the exposure of pregnant and lactating rats to Co resulted in the development of oxidative stress and the impairment of defense systems in the cerebrum and cerebellum of their suckling pups.

KeywordsCobalt chloride Suckling rats Cerebrum Cerebellum Antioxidant enzyme profiles Histological studies AbbreviationsAChEAcetylcholinesterase

AOPPAdvanced oxidation protein product

Bcl-xlB cell lymphoma-extra large

Bcl-2B cell lymphoma 2

BuChEButylcholinesterase

CATCatalase

CoCobalt

DTNB5,5′-Dithiobtis-2-nitrobenzoic acid

EGLExternal granular layer

GPxGlutathione peroxidase

GSHGlutathione

H2O2Hydrogen peroxide

IGLInternal granular layer

MDAMalondialdehyde

MLMolecular layer

NBTNitro blue tetrazolium

NPSHNonprotein thiol

PCLPurkinje cell layer

PCOProtein carbonyl

PUFAPolyunsaturated fatty acids

ROSReactive oxygen species

SODSuperoxide dismutase

Ibtissem Ben Amara and Elmouldi Garoui contributed equally to this work.

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Autor: Elmouldi Garoui - Ibtissem Ben Amara - Dorra Driss - Awatef Elwej - Semia Ellouze Chaabouni - Tahia Boudawara - Najiba Ze

Fuente: https://link.springer.com/







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