Effect of avian influenza A H5N1 infection on the expression of microRNA-141 in human respiratory epithelial cellsReportar como inadecuado




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BMC Microbiology

, 13:104

Microbe-host interactions and microbial pathogenicity

Abstract

BackgroundAvian influenza remains a serious threat to human health. The consequence of human infection varies markedly among different subtypes of avian influenza viruses. In addition to viral factors, the difference in host cellular response is likely to play a critical role. This study aims at elucidating how avian influenza infection perturbs the host’s miRNA regulatory pathways that may lead to adverse pathological events, such as cytokine storm, using the miRNA microarray approach.

ResultsThe results showed that dysregulation of miRNA expression was mainly observed in highly pathogenic avian influenza A H5N1 infection. We found that miR-21*, miR-100*, miR-141, miR-574-3p, miR-1274a and miR1274b were differentially expressed in response to influenza A virus infection. Interestingly, we demonstrated that miR-141, which was more highly induced by H5N1 than by H1N1 p < 0.05, had an ability to suppress the expression of a cytokine - transforming growth factor TGF-β2. This was supported by the observation that the inhibitory effect could be reversed by antagomiR-141.

ConclusionsSince TGF-β2 is an important cytokine that can act as both an immunosuppressive agent and a potent proinflammatory molecule through its ability to attract and regulate inflammatory molecules, and previous report showed that only seasonal influenza H1N1 but not the other avian influenza subtypes could induce a persistent expression of TGF-β2, we speculate that the modulation of TGF-β2 expression by different influenza subtypes via miR-141 might be a critical step for determining the outcome of either normal or excessive inflammation progression.

KeywordsmicroRNA Influenza A virus H1N1 H5N1 Inflammation Hypercytokinemia Pathogenesis Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-13-104 contains supplementary material, which is available to authorized users.

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Autor: Wai-Yip Lam - Apple Chung-Man Yeung - Karry Lei-Ka Ngai - Man-Shan Li - Ka-Fai To - Stephen Kwok-Wing Tsui - Paul Kay-Sh

Fuente: https://link.springer.com/







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