Mesenchymal Stem Cells Utilize CXCR4–SDF-1 Signaling for Acute, but Not Chronic, Trafficking to Gastric Mucosal InflammationReportar como inadecuado

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Digestive Diseases and Sciences

, Volume 58, Issue 9, pp 2466–2477

First Online: 20 July 2013Received: 25 April 2013Accepted: 25 June 2013DOI: 10.1007-s10620-013-2782-y

Cite this article as: Stoicov, C., Li, H., Liu, J.H. et al. Dig Dis Sci 2013 58: 2466. doi:10.1007-s10620-013-2782-y


BackgroundHelicobacter infection is the main risk factor in developing gastric cancer. Mesenchymal stem cells MSCs are non-hematopoietic stromal cells, which are able to differentiate into different cell lineages. MSC contribute to cancer development by forming the tumor directly, contributing to the microenvironment, or by promoting angiogenesis and metastasis. CXCR4-SDF-1 axis is used by MSC in trafficking, homing, and engraftment at chronic inflammation sites, and plays an important role in tumorigenesis.

AimTo determine if CXCR4 receptor has a role in MSC contribution to the development of Helicobacter-mediated gastric cancer.

MethodsSDF-1 and CXCR4 expression in mouse gastric mucosa in the setting of acute and chronic inflammation was measured using RT-PCR. Mouse culture-adapted MSC express CXCR4. Wild-type C57BL-6 mice infected with Helicobacter felis for 6 months or controls were given IV injections of CXCR4 knock-down MSC. Animals were followed for another 4 months. Homing of MSC in the stomach was quantified using RT-PCR. MSC differentiation into gastric epithelia lineages was analyzed using immunohistochemistry and fluorescent in situ hybridization.

ResultsCXCR4 and SDF-1 are both upregulated in the settings of Helicobacter-induced chronic gastric inflammation. CXCR4 is fully required for homing of MSC to the stomach in acute gastric inflammation, but only partially in Helicobacter-induced gastric cancer. MSC lead to gastric intraepithelial neoplasia as early as 10 months of Helicobacter infection.

ConclusionsOur results show that MSC have a tumorigenic effect by promoting an accelerated form of gastric cancer in mice. The engraftment of MSC in chronic inflammation is only partially CXCR4-dependent.

KeywordsGastric cancer Helicobacter Mesenchymal stem cells CXCR4 SDF-1  Download fulltext PDF

Autor: Calin Stoicov - Hanchen Li - Jian Hua Liu - JeanMarie Houghton


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