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BMC Genomics

, 14:322

Plant genomics

Abstract

BackgroundRoot-knot nematodes RKN– Meloidogyne genus present extensive challenges to soybean crop. The soybean line PI 595099 is known to be resistant against specific strains and races of nematode species, thus its differential gene expression analysis can lead to a comprehensive gene expression profiling in the incompatible soybean-RKN interaction. Even though many disease resistance genes have been studied, little has been reported about phytohormone crosstalk on modulation of ROS signaling during soybean-RKN interaction.

ResultsUsing 454 technology to explore the common aspects of resistance reaction during both parasitism and resistance phases it was verified that hormone, carbohydrate metabolism and stress related genes were consistently expressed at high levels in infected roots as compared to mock control. Most noteworthy genes include those encoding glycosyltransferases, peroxidases, auxin-responsive proteins and gibberellin-regulated genes. Our data analysis suggests the key role of glycosyltransferases, auxins and components of gibberellin signal transduction, biosynthesis and deactivation pathways in the resistance reaction and their participation in jasmonate signaling and redox homeostasis in mediating aspects of plant growth and responses to biotic stress.

ConclusionsBased on this study we suggest a reasonable model regarding to the complex mechanisms of crosstalk between plant hormones, mainly gibberellins and auxins, which can be crucial to modulate the levels of ROS in the resistance reaction to nematode invasion. The model also includes recent findings concerning to the participation of DELLA-like proteins and ROS signaling controlling plant immune or stress responses. Furthermore, this study provides a dataset of potential candidate genes involved in both nematode parasitism and resistance, which can be tested further for their role in this biological process using functional genomics approaches.

KeywordsRoot–knot nematode Glycine max Transcriptome Pyrosequencing Plant–pathogen interaction Hormone Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-14-322 contains supplementary material, which is available to authorized users.

Magda Aparecida Beneventi, Orzenil Bonfim da Silva Jr contributed equally to this work.

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Autor: Magda Aparecida Beneventi - Orzenil Bonfim da SilvaJr - Maria Eugênia Lisei de Sá - Alexandre Augusto Pereira Firmino

Fuente: https://link.springer.com/



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