Comparison of the effects of photon versus carbon ion irradiation when combined with chemotherapy in vitroReportar como inadecuado

Comparison of the effects of photon versus carbon ion irradiation when combined with chemotherapy in vitro - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Radiation Oncology

, 8:260

Radiation Biology and Molecular Radiation Oncology


BackgroundCharacterization of combination effects of chemotherapy drugs with carbon ions in comparison to photons in vitro.

MethodsThe human colon adenocarcinoma cell line WiDr was tested for combinations with camptothecin, cisplatin, gemcitabine and paclitaxel. In addition three other human tumour cell lines A549: lung, LN-229: glioblastoma, PANC-1: pancreas were tested for the combination with camptothecin. Cells were irradiated with photon doses of 2, 4, 6 and 8 Gy or carbon ion doses of 0.5, 1, 2 and 3 Gy. Cell survival was assessed using the clonogenic growth assay. Treatment dependent changes in cell cycle distribution up to 12 hours post-treatment were measured by FACS analysis after propidium-iodide staining. Apoptosis was monitored for up to 36 hours post-treatment by Nicoletti-assay with qualitative verification using DAPI staining.

ResultsAll cell lines exhibited the well-known increase of killing efficacy per unit dose of carbon ion exposure, with relative biological efficiencies at 10% survival RBE10 ranging from 2.3 to 3.7 for the different cell lines. In combination with chemotherapy additive toxicity was the prevailing effect. Only in combination with gemcitabine or cisplatin WiDr or camptothecin all cell lines the photon sensitivity was slightly enhanced, whereas purely independent toxicities were found with the carbon ion irradiation, in all cases. Radiation-induced cell cycle changes displayed the generally observed dose-dependent G2-arrest with little effect on S-phase fraction for all cell lines for photons and for carbon ions. Only paclitaxel showed a significant induction of apoptosis in WiDr cell line but independent of the used radiation quality.

ConclusionsCombined effects of different chemotherapeutics with photons or with carbon ions do neither display qualitative nor substantial quantitative differences. Small radiosensitizing effects, when observed with photons are decreased with carbon ions. The data support the idea that a radiochemotherapy with common drugs and carbon ion irradiation might be as feasible as respective photon-based protocols. The present data serve as an important radiobiological basis for further combination experiments, as well as clinical studies on combination treatments.

KeywordsHuman tumour cells Carbon ion radiotherapy Chemotherapy Clonogenic survival Electronic supplementary materialThe online version of this article doi:10.1186-1748-717X-8-260 contains supplementary material, which is available to authorized users.

Download fulltext PDF

Autor: Fabian Schlaich - Stephan Brons - Thomas Haberer - Jürgen Debus - Stephanie E Combs - Klaus Josef Weber


Documentos relacionados