Derivation of androgenetic embryonic stem cells from m-carboxycinnamic acid bishydroxamide CBHA treated androgenetic embryosReportar como inadecuado




Derivation of androgenetic embryonic stem cells from m-carboxycinnamic acid bishydroxamide CBHA treated androgenetic embryos - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Chinese Science Bulletin

, Volume 58, Issue 23, pp 2862–2868

First Online: 11 June 2013Received: 01 March 2013Accepted: 03 May 2013DOI: 10.1007-s11434-013-5921-0

Cite this article as: Shuai, L., Feng, C., Zhang, H. et al. Chin. Sci. Bull. 2013 58: 2862. doi:10.1007-s11434-013-5921-0

Abstract

The androgenetic embyronic stem aES cells are useful models in studying the effects of imprinted genes on pluripotency maintaining and embryo development. The expression patterns of imprinted genes are significantly different between uniparental derived aES cells and zygote-derived embryonic stem ES cells, therefore, the imprinting related cell pluripotency needs further exploitation. Several approaches have been applied in generation of androgenetic embryos and derivation of aES cell lines. Here, we describe a method to generate androgenetic embryos by injecting two mature sperms into one enucleated oocyte. Then these androgenetic embryos were treated with a histone deacetylase inhibitor: m-carboxycinnamic acid bishydroxamide CBHA. Further, aES cell lines were successfully derived from these treated androgenetic embryos at blastocyst stage. The CBHA could improve not only the quality of androgenetic embryos, but also the efficiencies of aES CaES cells derivation and chimeric mice generation. The imprinted gene expression pattern in the CBHA treated embryo-derived aES CaES cells was also highly similar to that of zygote-derived ES cells.

KeywordsCBHA androgenetic aES pluripotency These authors contributed equally to this work.

This article is published with open access at Springerlink.com

Electronic Supplementary MaterialSupplementary material is available for this article at 10.1007-s11434-013-5921-0 and is accessible for authorized users.

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Autor: Ling Shuai - ChunJing Feng - HaiJiang Zhang - Qi Gu - YunDan Jia - Liu Wang - Xiao-Yang Zhao - ZhongHua Liu - Qi Zhou

Fuente: https://link.springer.com/







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