Cell cycle regulator geminin is dispensable for the proliferation of vascular smooth muscle cellsReportar como inadecuado




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Science China Life Sciences

, Volume 56, Issue 8, pp 731–738

First Online: 10 July 2013Received: 28 January 2013Accepted: 04 June 2013DOI: 10.1007-s11427-013-4513-1

Cite this article as: Guo, J. & Sun, N. Sci. China Life Sci. 2013 56: 731. doi:10.1007-s11427-013-4513-1

Abstract

The proliferation of vascular smooth muscle cells VSMCs plays a major role in the pathogenesis of many cardiovascular diseases. Geminin regulates DNA replication and cell cycle progression and plays a key role in the proliferation of cancer cells. We therefore hypothesized that geminin regulates the proliferation of VSMCs. The present study demonstrates that the level of geminin expression was low in quiescent VSMCs approximately 90% and 10% of cells in the G1 and in S-G2-M phases of the cell cycle, respectively, increased as more cells entered in S-G2-M, and then decreased as cells exited S-G2-M. Further, angiotensin II and norepinephrine stimulated expression of geminin in VSMCs. However, the DNA content, nuclear morphology, percentage of cells at different stages of the cell cycle, and rate of proliferation of VSMCs from which geminin was either depleted or overexpressed were all similar. These findings indicate geminin functions differently in VSMCs than it does in cancer cell lines and that it may provide a target for treating cancers without affecting normal cells.

Keywordsgeminin DNA replication cell cycle proliferation vascular smooth muscle cell This article is published with open access at Springerlink.com

Electronic Supplementary MaterialSupplementary material is available for this article at 10.1007-s11427-013-4513-1 and is accessible for authorized users.

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Autor: JianQiang Guo - NingLing Sun

Fuente: https://link.springer.com/







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