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Journal of Hematology and Oncology

, 7:6

First Online: 10 January 2014Received: 05 December 2013Accepted: 04 January 2014DOI: 10.1186-1756-8722-7-6

Cite this article as: Wang, WT., Zhao, YN., Yan, JX. et al. J Hematol Oncol 2014 7: 6. doi:10.1186-1756-8722-7-6


BackgroundThe purpose of this study was to detect the serum microRNAs miRNAs that are differentially expressed in cervical squamous cell carcinoma SCC patients and negative controls, with a focus on the miRNA profiles of the patients before and after surgery. The aim of the study is to evaluate the potential of these miRNAs as novel markers for the post-therapeutic monitoring of cervical SCC patients.

ResultsA total of 765 serum miRNAs from 10 cervical SCC patients before surgery, 10 cervical SCC patients after surgery, and 10 negative controls were profiled using a TaqMan MicroRNA Array. A set of selected differentially expressed miRNAs were further analyzed in the patients at different perioperative periods, including preoperative, 1 week postoperative, and one month postoperative. The results showed that several serum miRNAs were differentially expressed in the cervical SCC patients compared with the negative controls, including miR-646, miR-141* and miR-542-3p. More importantly, we found that levels of specific serum miRNAs were deregulated in the pre- and postoperative stages, and these miRNAs could be useful for post-therapeutic monitoring of disease progression. Finally, we depicted a regulatory network of differentially expressed serum miRNAs, and many possible target genes were predicted in the estrogen-mediated signal pathways, supporting the hypothesis that cervical SCC is a hormone-associated gynecological disease.

ConclusionsOur study demonstrated that the circulating miRNAs miR-646, miR-141* and miR-542-3p could potentially serve as non-invasive biomarkers for cervical SCC. The levels of these specific miRNAs might be useful for the post-therapeutic monitoring of disease progression. This is the first report showing that circulating miRNAs could serve as biomarkers for the therapeutic intervention of cervical SCC.

KeywordsCirculating microRNA Cervical SCC Serum Tumor biomarkers Post-therapeutic monitoring AbbreviationsmiRNAmicroRNA

SCCSquamous cell carcinoma

HPVHuman papillomavirus

PCRPolymerse chin rection

RT-PCRReverse transcription-PCR


DAVIDDatabase for Annotation, Visualization and Integrated Discovery

IGF1RInsulin-like growth factor 1

FOXO1Orkhead box O1

ADAM9Metallopeptidase domain 9

ATCAnthracycline combination

SCCASquamous cell carcinoma antigen

CA125Carbohydrate antigen 125

VEGFVascular endothelial growth factor.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-8722-7-6 contains supplementary material, which is available to authorized users.

Wen-Tao Wang, Ya-Nan Zhao contributed equally to this work.

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Autor: Wen-Tao Wang - Ya-Nan Zhao - Jin-Xing Yan - Mei-Ying Weng - Yan Wang - Yue-Qin Chen - Shun-Jia Hong

Fuente: https://link.springer.com/

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