Leaf cDNA-AFLP analysis of two citrus species differing in manganese tolerance in response to long-term manganese-toxicityReport as inadecuate




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BMC Genomics

, 14:621

Plant genomics

Abstract

BackgroundVery little is known about manganese Mn-toxicity-responsive genes in citrus plants. Seedlings of ‘Xuegan’ Citrus sinensis and ‘Sour pummelo’ Citrus grandis were irrigated for 17 weeks with nutrient solution containing 2 μM control or 600 μM Mn-toxicity MnSO4. The objectives of this study were to understand the mechanisms of citrus Mn-tolerance and to identify differentially expressed genes, which might be involved in Mn-tolerance.

ResultsUnder Mn-toxicity, the majority of Mn in seedlings was retained in the roots; C. sinensis seedlings accumulated more Mn in roots and less Mn in shoots leaves than C. grandis ones and Mn concentration was lower in Mn-toxicity C. sinensis leaves compared to Mn-toxicity C. grandis ones. Mn-toxicity affected C. grandis seedling growth, leaf CO2 assimilation, total soluble concentration, phosphorus P and magenisum Mg more than C. sinensis. Using cDNA-AFLP, we isolated 42 up-regulated and 80 down-regulated genes in Mn-toxicity C. grandis leaves. They were grouped into the following functional categories: biological regulation and signal transduction, carbohydrate and energy metabolism, nucleic acid metabolism, protein metabolism, lipid metabolism, cell wall metabolism, stress responses and cell transport. However, only 7 up-regulated and 8 down-regulated genes were identified in Mn-toxicity C. sinensis ones. The responses of C. grandis leaves to Mn-toxicity might include following several aspects: 1 accelerating leaf senescence; 2 activating the metabolic pathway related to ATPase synthesis and reducing power production; 3 decreasing cell transport; 4 inhibiting protein and nucleic acid metabolisms; 5 impairing the formation of cell wall; and 6 triggering multiple signal transduction pathways. We also identified many new Mn-toxicity-responsive genes involved in biological and signal transduction, carbohydrate and protein metabolisms, stress responses and cell transport.

ConclusionsOur results demonstrated that C. sinensis was more tolerant to Mn-toxicity than C. grandis, and that Mn-toxicity affected gene expression far less in C. sinensis leaves. This might be associated with more Mn accumulation in roots and less Mn accumulation in leaves of Mn-toxicity C. sinensis seedlings than those of C. grandis seedlings. Our findings increase our understanding of the molecular mechanisms involved in the responses of plants to Mn-toxicity.

KeywordscDNA-AFLP Citrus grandis Citrus sinensis Leaves Manganese Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-14-621 contains supplementary material, which is available to authorized users.

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Author: Chen-Ping Zhou - Yi-Ping Qi - Xiang You - Lin-Tong Yang - Peng Guo - Xin Ye - Xin-Xing Zhou - Feng-Jiao Ke - Li-Song Chen

Source: https://link.springer.com/







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