Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In VitroReport as inadecuate




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International Journal of Cell BiologyVolume 2010 2010, Article ID 928169, 13 pages

Research Article

Departments of Pathology and Cell Biology, University of Montreal, 2900, Bd Edouard-Montpetit, Montreal, Canada H3T 1J4

Department of Nutrition, CHU-Sainte-Justine Research Center, University of Montreal, 2900, Bd Edouard-Montpetit, Montreal, Canada H3T 1J4

Received 14 December 2009; Accepted 11 February 2010

Academic Editor: Gary S. Stein

Copyright © 2010 Philippe G. Cammisotto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Gastric Leptin is absorbed by duodenal enterocytes and released on the basolateral side towards the bloodstream. We investigated in vitro some of the mechanisms of this transport. Caco-2-15 cells internalize leptin from the apical medium and release it through transcytosis in the basal medium in a time- temperature-dependent and saturable fashion. Leptin receptors are revealed on the apical brush-border membrane of the Caco-2 cells. RNA-mediated silencing of the receptor led to decreases in the uptake and basolateral release. Leptin in the basal medium was found bound to the soluble form of its receptor. An inhibitor of clathrin-dependent endocytosis chlorpromazine decreased leptin uptake. Confocal immunocytochemistry and the use of brefeldin A and okadaic acid revealed the passage of leptin through the Golgi apparatus. We propose that leptin transcytosis by intestinal cells depends on its receptor, on clathrin-coated vesicles and transits through the Golgi apparatus.





Author: Philippe G. Cammisotto, Moise Bendayan, Alain Sané, Michel Dominguez, Carole Garofalo, and Émile Levy

Source: https://www.hindawi.com/



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