Elementary Flux Mode Analysis of Acetyl-CoA Pathway in Carboxydothermus hydrogenoformans Z-2901Report as inadecuate

Elementary Flux Mode Analysis of Acetyl-CoA Pathway in Carboxydothermus hydrogenoformans Z-2901 - Download this document for free, or read online. Document in PDF available to download.

Advances in Bioinformatics - Volume 2014 2014, Article ID 928038, 10 pages -

Research ArticleBioinformatics Lab, AU-KBC Research Centre, M.I.T Campus of Anna University, Chromepet, Chennai, Tamil Nadu 600 044, India

Received 10 January 2014; Revised 10 March 2014; Accepted 14 March 2014; Published 16 April 2014

Academic Editor: Ming Chen

Copyright © 2014 Rajadurai Chinnasamy Perumal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Carboxydothermus hydrogenoformans is a carboxydotrophic hydrogenogenic bacterium species that produces hydrogen molecule by utilizing carbon monoxide CO or pyruvate as a carbon source. To investigate the underlying biochemical mechanism of hydrogen production, an elementary mode analysis of acetyl-CoA pathway was performed to determine the intermediate fluxes by combining linear programming LP method available in CellNetAnalyzer software. We hypothesized that addition of enzymes necessary for carbon monoxide fixation and pyruvate dissimilation would enhance the theoretical yield of hydrogen. An in silico gene knockout of pyk, pykC, and mdh genes of modeled acetyl-CoA pathway allows the maximum theoretical hydrogen yield of 47.62 mmol-gCDW-h for 1 mole of carbon monoxide CO uptake. The obtained hydrogen yield is comparatively two times greater than the previous experimental data. Therefore, it could be concluded that this elementary flux mode analysis is a crucial way to achieve efficient hydrogen production through acetyl-CoA pathway and act as a model for strain improvement.

Author: Rajadurai Chinnasamy Perumal, Ashok Selvaraj, and Gopal Ramesh Kumar

Source: https://www.hindawi.com/


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