FGF2-induced effects on transcriptome associated with regeneration competence in adult human fibroblastsReport as inadecuate




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BMC Genomics

, 14:656

Human and rodent genomics

Abstract

BackgroundAdult human fibroblasts grown in low oxygen and with FGF2 supplementation have the capacity to tip the healing outcome of skeletal muscle injury – by favoring regeneration response in vivo over scar formation. Here, we compare the transcriptomes of control adult human dermal fibroblasts and induced regeneration-competent iRC fibroblasts to identify transcriptional changes that may be related to their regeneration competence.

ResultsWe identified a unique gene-expression profile that characterizes FGF2-induced iRC fibroblast phenotype. Significantly differentially expressed genes due to FGF2 treatment were identified and analyzed to determine overrepresented Gene Ontology terms. Genes belonging to extracellular matrix components, adhesion molecules, matrix remodelling, cytoskeleton, and cytokines were determined to be affected by FGF2 treatment.

ConclusionsTranscriptome analysis comparing control adult human fibroblasts with FGF2-treated fibroblasts identified functional groups of genes that reflect transcriptional changes potentially contributing to their regeneration competence. This comparative transcriptome analysis should contribute new insights into genes that characterize cells with greater regenerative potential.

KeywordsTranscriptome Human fibroblasts Fibroblast growth factor FGF2 Wound healing Regeneration Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-14-656 contains supplementary material, which is available to authorized users.

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Author: Olga Kashpur - David LaPointe - Sakthikumar Ambady - Elizabeth F Ryder - Tanja Dominko

Source: https://link.springer.com/







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