Proteomic analysis of differentially expressed proteins in vitamin C-treated AGS cellsReport as inadecuate

Proteomic analysis of differentially expressed proteins in vitamin C-treated AGS cells - Download this document for free, or read online. Document in PDF available to download.

BMC Biochemistry

, 14:24

Protein and enzyme biochemistry


BackgroundVitamin C ascorbic acid is an essential nutrient of most living tissues that readily acts as a strong reducing agent, which is abundant in fruits and vegetables. Although, it inhibits cell growth in many human cancer cells in vitro, treatment in cancer is still controversial. Hence, the purpose of this study was to investigate the molecular mechanism of the inhibitory effect of vitamin C on AGS cell growth, and protein profiles in AGS cells after exposure to vitamin C treatment, by using proteomic tools.

ResultsVitamin C showed a cytotoxic effect on AGS cells IC50 300 μg-mL and, 20 differentially expressed proteins spot intensities which show ≥2 fold change and statistically significant, p<0.05 between the control and vitamin-C treated group were successfully identified by assisted laser desorption- ionization-time of flight-mass spectrometry MALDI-TOF-MS. Of the 20 proteins, six were up-regulated and fourteen were down-regulated. Specifically, 14-3-3σ, 14-3-3ϵ, 14-3-3δ, tropomyosin alpha-3 chain and tropomyosin alpha-4 chain were down-regulated and peroxiredoxin-4 and thioredoxin domain-containing proteins 5 were up-regulated. The identified proteins are mainly involved in cell mobility, antioxidant and detoxification, signal transduction and protein metabolism. Further, the expressions of 14-3-3 isoforms were verified with immuno-blotting analysis.

ConclusionsOur proteome results suggest that the apoptosis related proteins were involved in promoting and regulating cell death of AGS cells, and might be helpful to understand the molecular mechanism of vitamin C on AGS cell growth inhibition.

KeywordsVitamin C Gastric cancer AGS cells Proteome analysis 14-3-3 isoforms Electronic supplementary materialThe online version of this article doi:10.1186-1471-2091-14-24 contains supplementary material, which is available to authorized users.

Arulkumar Nagappan, Hyeon Soo Park contributed equally to this work.

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Author: Arulkumar Nagappan - Hyeon Soo Park - Kwang Il Park - Jin A Kim - Gyeong Eun Hong - Sang Rim Kang - Jue Zhang - Eun H


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