Mimotope vaccination for epitope-specific induction of anti-VEGF antibodiesReport as inadecuate




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BMC Biotechnology

, 13:77

Applied immunology

Abstract

BackgroundTumor angiogenesis is critical for tumor growth, infiltration and metastasis. Vascular endothelial growth factor VEGF is a potent angiogenic factor and targeting it is important in reducing angiogenesis. Bevacizumab Avastin, a monoclonal antibody that reacts directly against VEGF, has been demonstrated to be an effective treatment for various cancers such as rectal cancer, colon carcinoma, and non-small cell lung cancer, etc.

ResultsIn the current study, we used the phage display technique to generate mimotopes that complemented the screening Avastin antibody Ab. The candidate mimotopes of VEGF were isolated from a 12-mer peptide library. The phage displaying peptide DHTLYTPYHTHP designated as 12P exhibited high affinity to Avastin. The chemically synthesized 12P was conjugated to keyhole limpet hemocyanin KLH by glutaraldehyde GA to form vaccine KLH-12 peptide KLH-12P. This epitope vaccine significantly induced humoral immunity in mice. The blood serum from KLH-12P-immunized mice associated with VEGF and blocked its binding to VEGFR, thus inhibiting vascular endothelial cell proliferation and migration.

ConclusionsOur data indicate that the isolated mimotope 12P reported here could potentially elicit specific antibodies against VEGF and result in the induction of anti-angiogenesis responses.

KeywordsAvastin VEGF Cancer immunotherapy Mimotope Phage display library Electronic supplementary materialThe online version of this article doi:10.1186-1472-6750-13-77 contains supplementary material, which is available to authorized users.

Weina Li, Yonggang Ran contributed equally to this work.

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Author: Weina Li - Yonggang Ran - Meng Li - Kuo Zhang - Xin Qin - Xiaochang Xue - Cun Zhang - Qiang Hao - Wei Zhang - Yingqi Zha

Source: https://link.springer.com/







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