The effect of microdosimetric 12C6 heavy ion irradiation and Mg2 on canthaxanthin production in a novel strain of Dietzia natronolimnaeaReport as inadecuate




The effect of microdosimetric 12C6 heavy ion irradiation and Mg2 on canthaxanthin production in a novel strain of Dietzia natronolimnaea - Download this document for free, or read online. Document in PDF available to download.

BMC Microbiology

, 13:213

Applied microbiology

Abstract

BackgroundDietzia natronolimnaea is one of the most important bacterial bioresources for high efficiency canthaxanthin production. It produces the robust and stable pigment canthaxanthin, which is of special interest for the development of integrated biorefineries. Mutagenesis employing C irradiation is a novel technique commonly used to improve microorganism productivity. This study presents a promising route to obtaining the highest feasible levels of biomass dry weight BDW, and total canthaxanthin by using a microdosimetric model of C irradiation mutation in combination with the optimization of nutrient medium components.

ResultsThis work characterized the rate of both lethal and non-lethal dose mutations for C irradiation and the microdosimetric kinetic model using the model organism, D. natronolimnaea svgcc1.2736. Irradiation with C ions resulted in enhanced production of canthaxanthin, and is therefore an effective method for strain improvement of D. natronolimnaea svgcc1.2736. Based on these results an optimal dose of 0.5–4.5 Gy, Linear energy transfer LET of 80 keV μmand energy of 60 MeV u for C irradiation are ideal for optimum and specific production of canthaxanthin in the bacterium. Second-order empirical calculations displaying high R-squared 0.996 values between the responses and independent variables were derived from validation experiments using response surface methodology. The highest canthaxanthin yield 8.14 mg was obtained with an optimized growth medium containing 21.5 g L D-glucose, 23.5 g L mannose and 25 ppm Mg in 1 L with an irradiation dose of 4.5 Gy.

ConclusionsThe microdosimetric C irradiation model was an effective mutagenic technique for the strain improvement of D. natronolimnaea svgcc1.2736 specifically for enhanced canthaxanthin production. At the very least, random mutagenesis methods using Cions can be used as a first step in a combined approach with long-term continuous fermentation processes. Central composite design-response surface methodologies CCD-RSM were carried out to optimize the conditions for canthaxanthin yield. It was discovered D-glucose, Mg and mannose have significant influence on canthaxanthin biosynthesis and growth of the mutant strain.

KeywordsD. natronolimnaea svgcc1.2736 Microdosimetric C-ions Irradiation Productivity Canthaxanthin Response surface methodology AbbreviationsBDWBiomass dry weight

BDWIBiomass dry weight integral

CXCanthaxanthin

AT mediumPeptic digest of animal tissue 5.000 Gms Litre; Yeast extract 3.000 Gms Litre; Malt extract 3.000 Gms Litre; Dextrose 10.000 Gms Litre; Agar 20.000 Gms Litre

HIRFLHeavy ion research facility in lanzhou

RBERelative biological effectiveness

RSMResponse surface methodology

LETLinear energy transfer

MKMMicrodosimetric kinetic model

SOBPSpread-out Bragg peak

SDSurviving fraction

CTCarotenoid

HPLCHigh performance liquid chromatography

CCDCentral composite design

ANOVAAnalyzed by analysis of variance

TSTTransportation safety technologies.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-13-213 contains supplementary material, which is available to authorized users.

Xiang Zhou, Jia-Rong Xie, Lei Tao, Feng-Wu Zhao contributed equally to this work.

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Author: Xiang Zhou - Jia-Rong Xie - Lei Tao - Zhi-Jun Xin - Feng-Wu Zhao - Xi-Hong Lu - Mei-Rong Zhao - Liang Wang - Jian-Ping Li

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