Transcriptome sequencing and whole genome expression profiling of chrysanthemum under dehydration stressReport as inadecuate

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BMC Genomics

, 14:662

Plant genomics


BackgroundChrysanthemum is one of the most important ornamental crops in the world and drought stress seriously limits its production and distribution. In order to generate a functional genomics resource and obtain a deeper understanding of the molecular mechanisms regarding chrysanthemum responses to dehydration stress, we performed large-scale transcriptome sequencing of chrysanthemum plants under dehydration stress using the Illumina sequencing technology.

ResultsTwo cDNA libraries constructed from mRNAs of control and dehydration-treated seedlings were sequenced by Illumina technology. A total of more than 100 million reads were generated and de novo assembled into 98,180 unique transcripts which were further extensively annotated by comparing their sequencing to different protein databases. Biochemical pathways were predicted from these transcript sequences. Furthermore, we performed gene expression profiling analysis upon dehydration treatment in chrysanthemum and identified 8,558 dehydration-responsive unique transcripts, including 307 transcription factors and 229 protein kinases and many well-known stress responsive genes. Gene ontology GO term enrichment and biochemical pathway analyses showed that dehydration stress caused changes in hormone response, secondary and amino acid metabolism, and light and photoperiod response. These findings suggest that drought tolerance of chrysanthemum plants may be related to the regulation of hormone biosynthesis and signaling, reduction of oxidative damage, stabilization of cell proteins and structures, and maintenance of energy and carbon supply.

ConclusionsOur transcriptome sequences can provide a valuable resource for chrysanthemum breeding and research and novel insights into chrysanthemum responses to dehydration stress and offer candidate genes or markers that can be used to guide future studies attempting to breed drought tolerant chrysanthemum cultivars.

KeywordsChrysanthemum Dehydration stress Gene expression Pathways RNA-seq Transcriptome Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-14-662 contains supplementary material, which is available to authorized users.

Yanjie Xu, Shan Gao, Yingjie Yang contributed equally to this work.

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Author: Yanjie Xu - Shan Gao - Yingjie Yang - Mingyun Huang - Lina Cheng - Qian Wei - Zhangjun Fei - Junping Gao - Bo Hong


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