The impact of different ale brewer’s yeast strains on the proteome of immature beerReport as inadecuate

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BMC Microbiology

, 13:215

Microbial genetics, genomics and proteomics


BackgroundIt is well known that brewer’s yeast affects the taste and aroma of beer. However, the influence of brewer’s yeast on the protein composition of beer is currently unknown. In this study, changes of the proteome of immature beer, i.e. beer that has not been matured after fermentation, by ale brewer’s yeast strains with different abilities to degrade fermentable sugars were investigated.

ResultsBeers were fermented from standard hopped wort 13° Plato using two ale brewer’s yeast Saccharomyces cerevisiae strains with different attenuation degrees. Both immature beers had the same alcohol and protein concentrations. Immature beer and unfermented wort proteins were analysed by 2-DE and compared in order to determine protein changes arising from fermentation. Distinct protein spots in the beer and wort proteomes were identified using Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry MALDI-TOF-MS and MS-MS and revealed common beer proteins, such as lipid transfer proteins LTP1 and LTP2, protein Z and amylase-protease inhibitors. During fermentation, two protein spots, corresponding to LTP2, disappeared, while three protein spots were exclusively found in beer. These three proteins, all derived from yeast, were identified as cell wall associated proteins, that is Exg1 an exo-β-1,3-glucanase, Bgl2 an endo-β-1,2-glucanase, and Uth1 a cell wall biogenesis protein.

ConclusionYeast strain dependent changes in the immature beer proteome were identified, i.e. Bgl2 was present in beer brewed with KVL011, while lacking in WLP001 beer.

Keywords2-DE Beer Fermentation Brewer’s yeast Secreted proteins Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-13-215 contains supplementary material, which is available to authorized users.

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Author: Torben Sune Berner - Susanne Jacobsen - Nils Arneborg


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