The clinical value of the quantitative detection of four cancer-testis antigen genes in multiple myelomaReport as inadecuate




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Molecular Cancer

, 13:25

First Online: 05 February 2014Received: 18 September 2013Accepted: 20 December 2013DOI: 10.1186-1476-4598-13-25

Cite this article as: Zhang, Y., Bao, L., Lu, J. et al. Mol Cancer 2014 13: 25. doi:10.1186-1476-4598-13-25

Abstract

BackgroundCancer-testis CT antigen genes might promote the progression of multiple myeloma MM. CT antigens may act as diagnostic and prognostic markers in MM, but their expression levels and clinical implications in this disease are not fully understood. This study measured the expression levels of four CT antigen genes in Chinese patients with MM and explored their clinical implications.

MethodsReal-time quantitative polymerase chain reaction qPCR was used to quantify the expression of MAGE-C1-CT7, MAGE-A3, MAGE-C2-CT10 and SSX-2 mRNA in 256 bone marrow samples from 144 MM patients.

ResultsIn the newly diagnosed patients, the positive expression rates were 88.5% for MAGE-C1-CT7, 82.1% for MAGE-C2-CT10, 76.9% for MAGE-A3 and 25.6% for SSX-2. The expression levels and the number of co-expressed CT antigens correlated significantly with several clinical indicators, including the percentage of plasma cells infiltrating the bone marrow, abnormal chromosome karyotypes and the clinical course.

ConclusionMAGE-C1-CT7, MAGE-A3, MAGE-C2-CT10 and SSX-2 expression levels provide potentially effective clinical indicators for the auxiliary diagnosis and monitoring of treatment efficacy in MM.

KeywordsCancer-testis antigen gene Multiple myeloma Real-time quantitative polymerase chain reaction Electronic supplementary materialThe online version of this article doi:10.1186-1476-4598-13-25 contains supplementary material, which is available to authorized users.

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Author: Yao Zhang - Li Bao - Jin Lu - Kai-Yan Liu - Jin-Lan Li - Ya-Zhen Qin - Huan Chen - Ling-Di Li - Yuan Kong - Hong-Xia Shi

Source: https://link.springer.com/







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