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BMC Genomics

, 14:669

Prokaryote microbial genomics

Abstract

BackgroundElucidating gut microbiota among gallstone patients as well as the complex bacterial colonization of cholesterol gallstones may help in both the prediction and subsequent lowered risk of cholelithiasis. To this end, we studied the composition of bacterial communities of gut, bile, and gallstones from 29 gallstone patients as well as the gut of 38 normal individuals, examining and analyzing some 299, 217 bacterial 16S rRNA gene sequences from 120 samples.

ResultsFirst, as compared with normal individuals, in gallstone patients there were significant P < 0.001 increases of gut bacterial phylum Proteobacteria and decreases of three gut bacterial genera, Faecalibacterium, Lachnospira, and Roseburia. Second, about 70% of gut bacterial operational taxonomic units OTUs from gallstone patients were detectable in the biliary tract and bacteria diversity of biliary tract was significantly P < 0.001 higher than that of gut. Third, analysis of the biliary tract core microbiome represented by 106 bacteria OTUs among gallstone patients showed that 33.96% 36-106 of constituents can be matched to known bacterial species 15 of which have publicly available genomes. A genome-wide search of MDR, BSH, bG, and phL genes purpotedly associated with the formation of cholesterol gallstones showed that all 15 species with known genomes e.g., Propionibacterium acnes, Bacteroides vulgates, and Pseudomonas putida contained at least contained one of the four genes. This finding could potentially provide underlying information needed to explain the association between biliary tract microbiota and the formation of cholesterol gallstones.

ConclusionsTo the best of our knowledge, this is the first study to discover gut microbiota dysbiosis among gallstone patients, the presence of which may be a key contributor to the complex bacteria community assembly linked with the presence of cholesterol gallstones. Likewise, this study also provides the first large-scale glimpse of biliary tract microbiota potentially associated with cholesterol gallstones. Such a characterization of the biliary tract core microbiome has potentially important biological and medical implications regarding the role of bacteria in the formation cholesterol gallstones.

KeywordsGut microbiota dysbiosis Cholesterol gallstone Bile Bacterial colonization Pyrosequencing AbbreviationsbGβ-glucuronidase

BSHBile acid hydrolases

CDCrohn’s disease

GITGastrointestinal tract

MDRMultidrug-resistance efflux pump proteins

OTUsOperational taxonomic units

phLPhospholipases

rRNARibosomal RNA.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-14-669 contains supplementary material, which is available to authorized users.

Tao Wu, Zhigang Zhang contributed equally to this work.

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Author: Tao Wu - Zhigang Zhang - Bin Liu - Dezhi Hou - Yun Liang - Jie Zhang - Peng Shi

Source: https://link.springer.com/







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