The expression of diacylglycerol kinase theta during the organogenesis of mouse embryosReport as inadecuate




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BMC Developmental Biology

, 13:35

Organogenesis

Abstract

BackgroundDiacylglycerol kinase DGK is a key enzyme that regulates diacylglycerol DG turnover and is involved in a variety of physiological functions. The isoform DGKθ has a unique domain structure and is the sole member of type V DGK. To reveal the spatial and temporal expression of DGKθ we performed immunohistochemical staining on paraffin sections of mouse embryos.

ResultsAt an early stage of development E10.5 and 11.5, the expression of DGKθ was prominently detected in the brain, spinal cord, dorsal root ganglion, and limb bud, and was also moderately detected in the bulbus cordis and the primordium of the liver and gut. At later stages E12.5 and 14.5, DGKθ expression persisted or increased in the neocortex, epithalamus, hypothalamus, medulla oblongata, and pons. DGKθ was also evident in the epidermis, and nearly all epithelia of the oropharyngeal membrane, digestive tract, and bronchea. At prenatal developmental stages E16.5 and E18.5, the expression pattern of DGKθ was maintained in the central nervous system, intestine, and kidney, but was attenuated in the differentiated epidermis.

ConclusionThese results suggest that DGKθ may play important physiological roles not only in the brain, but also in diverse organs and tissues during the embryonic stages.

KeywordsDiacylglycerol kinase Embryonic development Central nerve system Epidermis Electronic supplementary materialThe online version of this article doi:10.1186-1471-213X-13-35 contains supplementary material, which is available to authorized users.

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Author: Shuji Ueda - Becky Tu-Sekine - Minoru Yamanoue - Daniel M Raben - Yasuhito Shirai

Source: https://link.springer.com/







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