Treatment of PC-3 cells with ultrasound combined with microbubbles induces distinct alterations in the expression of Bcl-2 and BaxReport as inadecuate




Treatment of PC-3 cells with ultrasound combined with microbubbles induces distinct alterations in the expression of Bcl-2 and Bax - Download this document for free, or read online. Document in PDF available to download.

Chinese Science Bulletin

, Volume 58, Issue 28–29, pp 3535–3540

First Online: 23 May 2013Received: 02 October 2012Accepted: 17 November 2012DOI: 10.1007-s11434-013-5753-y

Cite this article as: Bai, W., Yang, S., Shen, E. et al. Chin. Sci. Bull. 2013 58: 3535. doi:10.1007-s11434-013-5753-y

Abstract

The objective of the present study was to investigate whether ultrasound combined with microbubbles induces apoptotic cell death in androgen-independent prostate cancer cells and to identify the probable mechanism. We used ultrasound in continuous wave mode with a frequency of 21 kHz and a spatial-average temporal-average intensity of 46 mW-cm. Ultrasound combined with microbubbles 200 μL SonoVue™ was used to treat androgen-independent human prostate cancer PC-3 cells for 30 s. PC-3 cells were divided into three groups: the control group, the ultrasound group and the ultrasound combined with microbubbles group. Immediately after treatment, trypan blue exclusion was used to assess cell viability. Cell apoptosis at 24 h after treatment was measured using transmission electron microscopy and flow cytometry. Western blotting was used to evaluate the expression of the apoptosis-related proteins, Bcl-2 and Bax. Ultrasound combined with microbubbles had a minimal effect on the viability of PC-3 cells and induced minimal levels of cell lysis. The level of apoptosis in PC-3 cells induced by this modality was significantly higher than in controls 12.77 ± 0.31% vs. 2.56 ± 0.22%, P<0.01. Treatment with ultrasound combined with microbubbles increased the expression of Bax, a pro-apoptotic protein, and decreased the expression of Bcl-2, an anti-apoptotic protein. It was concluded that ultrasound combined with microbubbles induces apoptotic cell death in human prostate cancer PC-3 cells through down-regulation of Bcl-2 and up-regulation of Bax.

Keywordslow-frequency ultrasound low-energy ultrasound microbubble apoptosis Bcl-2 Bax This article is published with open access at Springerlink.com

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Author: WenKun Bai - ShaoLing Yang - E. Shen - JiZhen Zhang - ZhiYong Shen - Bing Hu

Source: https://link.springer.com/







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