Performance of five molecular methods for monitoring Arcobacter sppReport as inadecuate

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BMC Microbiology

, 13:220

Applied microbiology


BackgroundBacteria belonging to the Arcobacter genus are emerging enteropathogens and potential zoonotic agents. Their taxonomy has evolved very rapidly, and there are presently 18 recorded species. The prevalence of species belonging to Arcobacter is underestimated because of the limitations of currently available methods for species identification.

The aim of this study was to compare the performance of five PCR based methods that target regions of 16S rRNA, 23S rRNA or gyrA genes to identify Arcobacter species, and to review previous results reported in the literature using these methods.

ResultsThe five tested methods were found not to be reliable. They misidentified between 16.8% and 67.4% of the studied strains; this was dependent upon the target regions of the tested genes. The worst results obtained were for the identification of Arcobacter cryaerophilus and Arcobacter butzleri when the 23S rRNA gene was used as the target. These species were confused with many non-targeted species.

ConclusionOur results suggest that the known diversity of Arcobacter spp. in different environments could be expanded if reliable identification methods are applied in future studies.

KeywordsArcobacter Identification Comparison Molecular methods 16S rRNA-RFLP m-PCR 23S rRNA gyrA Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-13-220 contains supplementary material, which is available to authorized users.

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Author: Arturo Levican - María José Figueras


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