Tracing the location of powdery mildew resistance-related gene Stpk-V by FISH with a TAC clone in Triticum aestivum-Haynaldia villosa alien chromosome linesReportar como inadecuado




Tracing the location of powdery mildew resistance-related gene Stpk-V by FISH with a TAC clone in Triticum aestivum-Haynaldia villosa alien chromosome lines - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Chinese Science Bulletin

, Volume 58, Issue 33, pp 4084–4091

First Online: 24 July 2013Received: 09 November 2012Accepted: 23 January 2013DOI: 10.1007-s11434-013-5851-x

Cite this article as: Yang, X., Cao, A., Sun, Y. et al. Chin. Sci. Bull. 2013 58: 4084. doi:10.1007-s11434-013-5851-x

Abstract

Bacterial artificial chromosomes BACs or yeast artificial chromosomes YACs containing large inserts as probes for fluorescence in situ hybridization FISH have been used in the physical mapping of specific DNA sequences, especially for single- or low-copy sequences. Our earlier study identified Stpk-V, a powdery mildew resistance-related gene located on the 6VS chromosome arm of the wild grass Haynaldia villosa tribe Triticeae, and obtained several Triticum aestivum-H. villosa alien chromosome lines carrying the Stpk-V gene. However, the precise physical location of the Stpk-V gene on chromosome 6VS is not known. In this study, we used TAC-FISH with TAC15 as the probe coupled with sequential genomic in situ hybridization GISH to determine the physical location of the Stpk-V gene in different T. aestivum-H. villosa 6V alien chromosome lines, including addition, substitution and translocation lines. The result indicated that the fraction length of the Stpk-V locus is 0.575±0.035 on the 6V chromosome short arm and this was confirmed by FISH using TAC15 as the probe for tracing the Stpk-V gene in other genetic stocks. The cytological mapping strategies used in this study will be of benefit for tracing the alien gene location in the course of introducing desirable traits from wild species.

Keywordsfluorescence in situ hybridization FISH transformation-competent artificial chromosome TAC serine-threonine protein kinase gene from the V-genome Stpk-V Triticum aestivum-Haynaldia villosa alien chromosome lines This article is published with open access at Springerlink.com

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Autor: XueMing Yang - AiZhong Cao - YuLei Sun - PeiDu Chen

Fuente: https://link.springer.com/







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