Epigallocatechin Gallate Does Not Accelerate the Early Phase of Liver Regeneration After Partial Hepatectomy in RatsReportar como inadecuado




Epigallocatechin Gallate Does Not Accelerate the Early Phase of Liver Regeneration After Partial Hepatectomy in Rats - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Digestive Diseases and Sciences

, Volume 59, Issue 5, pp 976–985

First Online: 08 December 2013Received: 01 July 2013Accepted: 15 November 2013DOI: 10.1007-s10620-013-2966-5

Cite this article as: Mezera, V., Kučera, O., Moravcová, A. et al. Dig Dis Sci 2014 59: 976. doi:10.1007-s10620-013-2966-5

Abstract

BackgroundTwo-thirds partial hepatectomy PHx is an established model for the study of liver regeneration after resection. This process is accompanied by oxidative stress.

AimsIn our study, we tested the effect of epigallocatechin gallate EGCG, a green tea antioxidant, on the early phase of liver regeneration after PHx.

MethodsMale Wistar rats were divided into five groups: I laparotomy + water for intraperitoneal injections, II laparotomy + EGCG 50 mg-kg body weight, III PHx + water for injections, IV PHx + EGCG 20 mg-kg and V PHx + EGCG 50 mg-kg, for 3 consecutive days. The rats were killed 24 h after surgery. Biochemical analysis of rat sera was performed. Histological samples were stained with hematoxylin and eosin and bromodeoxyuridine BrdU. In hepatectomized rats, we also measured plasma malondialdehyde, tissue malondialdehyde, glutathione and cytokines levels, the activity of caspases 3-7, expression of Nqo-1 and HO-1 genes at the mRNA level, and expression of p21, p-p27 and p-p53 genes at the protein level.

ResultsWe observed lower accumulation of BrdU in group V when compared to groups III and IV. The activity of caspases 3-7 and expression of p-p53 were lower in group V than in groups III and IV. Tissue levels of IL-6 were lower in group V when compared to group III. Significant differences were not noted in other parameters.

ConclusionsAdministration of EGCG did not stimulate early phase liver regeneration in rats after PHx. There was even lower DNA synthesis in the group treated with a high dose of EGCG.

KeywordsAntioxidants Epigallocatechin gallate Hepatectomy Liver regeneration Oxidative stress AbbreviationsAIWater for injections

ALTAlanine aminotransferase

ALPAlkaline phosphatase

ASTAspartate aminotransferase

BrdU5-Bromo-2-deoxyuridine

EGCGEpigallocatechin gallate

EGCG20Epigallocatechin gallate 20 mg-kg body weight

EGCG50Epigallocatechin gallate 50 mg-kg body weight

GSHGlutathione reduced form

GSSGGlutathione oxidized form

HO-1Heme oxygenase-1

HRRHepatic regeneration rate 25

IL-6Interleukin-6

LAPLaparotomy

LRRLiver regeneration rate 26

MDAMalondialdehyde

Nqo-1NADPH-quinone-oxidoreductase

Nrf2-2NF-E2-related factor 2

PHxTwo-thirds partial hepatectomy

TGF-β1Transforming growth factor beta1

TNF-αTumor necrosis factor alpha

Download fulltext PDF



Autor: Vojtěch Mezera - Otto Kučera - Alena Moravcová - Eva Peterová - Zuzana Červinková

Fuente: https://link.springer.com/







Documentos relacionados