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Science China Life Sciences

, Volume 57, Issue 2, pp 162–170

First Online: 17 January 2014Received: 17 August 2013Accepted: 20 September 2013DOI: 10.1007-s11427-013-4598-6

Cite this article as: Huang, K., Liu, P., Li, X. et al. Sci. China Life Sci. 2014 57: 162. doi:10.1007-s11427-013-4598-6

Abstract

The breakthrough development of induced pluripotent stem cells iPSCs raises the prospect of patient-specific treatment for many diseases through the replacement of affected cells. However, whether iPSC-derived functional cell lineages generate a deleterious immune response upon auto-transplantation remains unclear. In this study, we differentiated five human iPSC lines from skin fibroblasts and urine cells into neural progenitor cells NPCs and analyzed their immunogenicity. Through co-culture with autogenous peripheral blood mononuclear cells PBMCs, we showed that both somatic cells and iPSC-derived NPCs do not stimulate significant autogenous PBMC proliferation. However, a significant immune reaction was detected when these cells were co-cultured with allogenous PBMCs. Furthermore, no significant expression of perforin or granzyme B was detected following stimulation of autogenous immune effector cells CD3CD8 T cells, CD3CD8 T cells or CD3CD56 NK cells by NPCs in both PBMC and T cell co-culture systems. These results suggest that human iPSC-derived NPCs may not initiate an immune response in autogenous transplants, and thus set a base for further preclinical evaluation of human iPSCs.

Keywordsinduced pluripotent stem cells immunogenicity iPSC-derived neural progenitor cells Contributed equally to this work

This article is published with open access at link.springer.com

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Autor: Ke Huang - PengFei Liu - Xiang Li - ShuBin Chen - LiHui Wang - Li Qin - ZhengHui Su - WenHao Huang - JuLi Liu - Bei Jia

Fuente: https://link.springer.com/



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