Genetic mapping of a 7R Al tolerance QTL in triticale x Triticosecale WittmackReportar como inadecuado

Genetic mapping of a 7R Al tolerance QTL in triticale x Triticosecale Wittmack - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Applied Genetics

, Volume 55, Issue 1, pp 1–14

First Online: 13 November 2013Received: 11 June 2013Revised: 03 September 2013Accepted: 04 September 2013DOI: 10.1007-s13353-013-0170-0

Cite this article as: Niedziela, A., Bednarek, P.T., Labudda, M. et al. J Appl Genetics 2014 55: 1. doi:10.1007-s13353-013-0170-0


Triticale x Triticosecale Wittmack is a relatively new cereal crop. In Poland, triticale is grown on 12 % of arable land There is an increasing interest in its cultivation due to lowered production costs and increased adaptation to adverse environmental conditions. However, it has an insufficient tolerance to the presence of aluminum ions Al in the soil. The number of genes controlling aluminum tolerance in triticale and their chromosomal location is not known. Two F2 mapping biparental populations MP1 and MP15 segregating for aluminum Al tolerance were tested with AFLP, SSR, DArT, and specific PCR markers. Genetic mapping enabled the construction of linkage groups representing chromosomes 7R, 5R and 2B. Obtained linkage groups were common for both mapping populations and mostly included the same markers. Composite interval mapping CIM allowed identification of a single QTL that mapped to the 7R chromosome and explained 25 % MP1 and 36 % MP15 of phenotypic variation. The B1, B26 and Xscm150 markers were 0.04 cM and 0.02 cM from the maximum of the LOD function in the MP1 and MP15, respectively and were highly associated with aluminum tolerance as indicated by Kruskal–Wallis nonparametric test. Moreover, the molecular markers B1, B26, Xrems1162 and Xscm92, previously associated with the Alt4 locus that encoded an aluminum-activated malate transporter ScALMT1 that was involved in Al tolerance in rye Secale cereale also mapped within QTL. Biochemical analysis of plants represented MP1 and MP15 mapping populations confirmed that the QTL located on 7R chromosome in both mapping populations is responsible for Al tolerance.

KeywordsAluminum tolerance Composite interval mapping Triticale AbbreviationsAFLPAmplified fragment length polymorphism

ALMTAluminum-activated malate transporter

DArTDiversity arrays technology

CIMComposite interval mapping

DHDoubled haploid

GWMGatersleben wheat microsatellites

LODLogarithm of odds

MATEMultidrug and toxin efflux

QTLQuantitative trait locus

PCRPolymerase chain reaction

REMSRye expressed microsatellite sites

SCMSecale cereale microsatellites

SSRSimple sequence repeat

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Autor: A. Niedziela - P. T. Bednarek - M. Labudda - D. R. Mańkowski - A. Anioł


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